Cellular and Growth-Factor Requirements for the Replication of Human Herpesvirus 6 in Primary Lymphocyte Cultures

  • Niza Frenkel
  • Ehud Roffman
  • Eric C. Schirmer
  • George Katsafanas
  • Linda S. Wyatt
  • Carl H. June
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 278)


Human herpesvirus 6 (HHV-6) was first isolated in 1986 from peripheral blood of AIDS patients and patients with lymphoproliferative disorders (1). Yamanishi and coworkers (2, 3) have further shown that HHV-6 is the causative agent of exanthem subitum (roseola infantum), a common childhood disease characterized by high fever and skin rash. Analyses of HHV-6 cell tropism have yielded complex results. The GS strain isolated by Salahuddin et al. (1) was shown to replicate in a variety of established cell lines including B cells positive for Epstein-Barr virus, continuous T-cell lines, megakaryocytes, and glioblastoma cells (4). Other HHV-6 strains were reported to replicate in continuous T-cell lines (5, 6). In contrast, the Z29 strain does not readily replicate in these cells (7, 8). While more quantitative studies are needed to evaluate the relative efficiencies of virus replication in different cell types, it is commonly accepted that HHV-6 isolates readily replicate in T cells (5–7, 9). Moreover, Takahashi et al. (10) reported that HHV-6 isolates infect predominantly CD4+ T cells in the peripheral-blood lymphocytes (PBL) of exanthem subitum patients.


Virus Replication Viral Structure 2Naval Medical Research Institute Mature Thymocyte Common Childhood Disease 


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  1. 1.
    Salahudin, S. Z., Ablashi, D. V., Markham, P. D., Josephs, S. F., Sturzenegger, Sturzenegger, S., Kaplan, M., Halligan, G., Biberfeld., Wong-Staal, F., Kramarsky, B. and Callo, R. C. (1986) Science (Washington, D.C., 1883-) 234, 596–601.CrossRefGoogle Scholar
  2. 2.
    Yamanishi, K., Shiraki, K., Kondo, T., Okuno, T., Takahashi, M., Asano, Y. and Kurata, T. (1988) Lancet 1, 1065–1067.PubMedCrossRefGoogle Scholar
  3. 3.
    Ueda, K., Kusuhara, K., Hirose, M., Okada, K., Miyazaki, C., Tokugawa, K., Nakayama, M. and Yamanishi, K. (1989) J. Infect. Dis. 159, 750–752.PubMedCrossRefGoogle Scholar
  4. 4.
    Ablashi, D. V., Lusso, P., Hung, C.-L., Salahuddin, S. Z., Josephs, S. F., Llana, T., Kramarsky, B., Biberfeld, P., Markham, P. D. and Gallo, R. C. (1988) Int. J. Cancer 42, 787–791.PubMedCrossRefGoogle Scholar
  5. 5.
    Downing, R. G., Sewankambo, N., Serwadda, D., Honess, R., Crawford, D., Jarrett, R. and Griffin, B. E. (1987) Lancet 2, 390.PubMedCrossRefGoogle Scholar
  6. 6.
    Tedder, R. S., Briggs, M., Cameron, C. H., Honess, R., Robertson, D. and Whittle, H. (1987) Lancet 2, 390–392.PubMedCrossRefGoogle Scholar
  7. 7.
    Lopez, C., Pellett, P., Stewart, J., Goldsmith, C., Sanderlin, K., Black, J., Warfield, D. and Feorino, P. (1988) J. Infect. Dis. 157, 1271–1273.PubMedCrossRefGoogle Scholar
  8. 8.
    Black, J. B., Sanderlin, K. C., Goldsmith, C. S., Gary, H. E., Lopez, C. and Pellett, P. (1989) J. Virol. Methods 26, 133–146.PubMedCrossRefGoogle Scholar
  9. 9.
    Lusso, P., Ensoli, B., Markham, P. D., Ablashi, D. V., Salahuddin, S. Z., Tschachler, E., Wong-Staal, F. and Gallo, R. C. (1989) Nature (London) 337, 370–373.CrossRefGoogle Scholar
  10. 10.
    Takahashi, K., Sonoda, S., Higashi, K., Kondo, T., Takahashi, H., Takahashi, M. and Yamanishi, K. (1989) J. Virol. 63, 3161–3163.PubMedGoogle Scholar
  11. 11.
    Balachandran, N., Ameise, R. E., Zhou, W. W. and Chang, C. K. (1989) J. Virol. 63, 2835–2840.PubMedGoogle Scholar
  12. 12.
    Roffman, E. and Frenkel, N. (1990) Virology 175, 591–594.PubMedCrossRefGoogle Scholar
  13. 13.
    Crabtree, G. R. (1989) Science (Washington, D.C., 1883-) 243, 355–361.CrossRefGoogle Scholar
  14. 14.
    Smith, K. A. (1988) Science (Washington, D.C., 1883-) 240, 1169–1176.CrossRefGoogle Scholar
  15. Margolick, S. B Willman, D. J., Folks, T. M. and Fauci, A. S. (1987) J. Immunol. 138 1719–1723.Google Scholar
  16. 16.
    Nabel, G. and Baltimore, D. (1987) Nature (London) 326, 711–713.CrossRefGoogle Scholar
  17. 17.
    Gowda, S. D., Stein, B. S., Mohagheghpour, N., Benike, C. J. and Engleman, E. J. (1989) J. Immunol. 142, 773–780.PubMedGoogle Scholar
  18. 18.
    Tong-Starksen, S. E., Luciw, P. A. and Peterlin, B. M. (1987) Proc. Natl. Acad Sci. USA 84, 6845–6849.PubMedCrossRefGoogle Scholar
  19. 19.
    Griffin, G. E., Kwanyee, L., Folks, T. M., Kunkel, S. and Nabel, G. J. (1989) Nature (London) 339, 70–73.CrossRefGoogle Scholar
  20. 20.
    Braun, R. W. and Reiser, H. C. (1986) J. Virol. 60, 29–36.PubMedGoogle Scholar
  21. 21.
    Sambucetti, L. C., Cherrington, J. M., Wilkinson, G. W. G. and Mocarski, E. S. (1989) EMBO J. 8, 4251–4258.PubMedGoogle Scholar
  22. 22.
    Hunninghake, G. W., Monick, M. M., Liu, B. and Stinski, M. F. (1989) J. Virol. 63, 3026–3033.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Niza Frenkel
    • 1
  • Ehud Roffman
    • 1
  • Eric C. Schirmer
    • 1
  • George Katsafanas
    • 1
  • Linda S. Wyatt
    • 1
  • Carl H. June
    • 2
  1. 1.Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases/TwinbrookNational Institutes of HealthRockvilleUSA
  2. 2.Naval Medical Research InstituteBethesdaUSA

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