Assessment of the Mutagenicity of Volatile Organic Air Pollutants Before and After Atmospheric Transformation

  • Larry D. Claxton
  • T. E. Kleindienst
  • Erica Perry
  • Larry T. Cupitt
Part of the Environmental Science Research book series (ESRH, volume 39)


During the past decade, renewed efforts have emerged to examine the extent to which hazardous compounds (particularly mutagens and carcinogens) are found in the urban atmosphere (20). The majority of these studies examined the organic material associated with particles emitted from specific sources (11). In contrast, only a few studies have examined volatile airborne organic compounds before and after they undergo atmospheric transformation (13). Some studies have shown that the mutagenicity of organic material from combustion sources was altered dramatically by photooxidation processes (1). Such studies also demonstrated that a variety of atmospheric hydrocarbons can be transformed into mutagenic species through these same processes (18). In this chapter, we present an overview of efforts used to assess the effect of atmospheric transformation upon the mutagenicity of airborne compounds.


Polycyclic Aromatic Hydrocarbon Mutagenic Activity Allyl Chloride Wood Smoke Atmospheric Reaction 
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  1. 1.
    Claxton, L.D., and M. Barnes (1981) The mutagenicity of diesel-exhaust particle extracts collected under smog-chamber conditions using the Salmonellatyphimurium test system. Mutat. Res. 88:255–272.PubMedCrossRefGoogle Scholar
  2. 2.
    Driver, J.H. (1988) Risk Assessment of Medical/Pathological Waste Incineration: Mutagenicity of Combustion Emissions. Ph.D. Thesis, University of North Carolina, Chapel Hill, North Carolina, 187 pp.Google Scholar
  3. 3.
    Driver, J.H., H.W. Rogers, and L.D. Claxton (1989) Mutagenicity of combustion emissions from a biomedical waste incinerator. Waste Management (in press).Google Scholar
  4. 4.
    Duce, R.A. (1978) Speculations on the budget of particulate and vapor-phase nonmethane organic carbon in the global troposphere. Pure Appl. Geophys. 116:244–273.CrossRefGoogle Scholar
  5. 5.
    Dumdei, B.E., D.V. Kenny, P.B. Shepson, T.E. Kleindienst, CM. Nero, L.T. Cupitt, and L.D. Claxton (1988) MS/MS analysis of the products of toluene photooxidation and measurement of their mutagenic activity. Env. Sci. and Tech. 22:1493–1498.CrossRefGoogle Scholar
  6. 6.
    Gibson, T.L., P.E. Korsog, and G.T. Wolff (1985) Evidence for the transformation of polycyclic organic matter in the atmosphere. Atmos. Env. 20:1575–1578.Google Scholar
  7. 7.
    Hughes, T.J., D.M. Simmons, L.G. Monteith, and L.D. Claxton (1987) Vaporization technique to measure mutagenic activity of volatile organic chemicals in the Ames/Salmonella assay. Env. Mutagen. 9:421–441.CrossRefGoogle Scholar
  8. 8.
    Kleindienst, T.E., P.B. Shepson, E.O. Edney, L.T. Cupitt, and L.D. Claxton (1985) The mutagenic activity of the products of propylene photooxidation. Env. Sci. Tech. 19:620–627.CrossRefGoogle Scholar
  9. 9.
    Kleindienst, T.E., P.B. Shepson, E.O. Edney, L.D. Claxton, and L.T. Cupitt (1986) Wood smoke: Measurement of the mutagenic activities of its gas- and particulate-phase photooxidation products. Env. Sci. Tech. 20:493–501.CrossRefGoogle Scholar
  10. 10.
    Leiter, J., M.B. Shimkin, and M.J. Shear (1942) Production of subcutaneous sarcomas in mice with tars extracted from atmospheric dusts. JNCI 3:155–165.Google Scholar
  11. 11.
    Lewtas, J. (1988) Genotoxicity of complex mixtures: Strategies for the identification and comparative assessment of airborne mutagens and carcinogens from combustion sources. Fund, and Appl. Tox. 10:571–589.CrossRefGoogle Scholar
  12. 12.
    Pitts, J.N., Jr. (1979) Photochemical and biological implications of the atmospheric reactions of amines and benzo(a)pyrene. Phil. Trans. R. Soc. Lond. A. 290:551–576.CrossRefGoogle Scholar
  13. 13.
    Pitts, J.N., Jr. (1983) Formation and fate of gaseous and particulate mutagens and carcinogens in real and simulated atmospheres. Env. Health Persp. 47:115–140.CrossRefGoogle Scholar
  14. 14.
    Pitts, J.N., Jr., K.A. Van Cauwenberghe, D. Grosjean, J. P. Schmid, D.R. Fitz, W.L. Belser Jr., G.B. Knudson, and P.M. Hynds (1978) Atmospheric reactions of polycyclic aromatic hydrocarbons: Facile formation of mutagenic nitro derivates. Science 202:515–519.PubMedCrossRefGoogle Scholar
  15. 15.
    Ramdahl, T., B. Zielinska, J. Arey, R. Atkinson, A.M. Winer, and J.N. Pitts, Jr. (1986) Ubiquitous occurrence of 2-nitrofluoranthene and 2-nitropyrene in air. Nature 321:425–427.PubMedCrossRefGoogle Scholar
  16. 16.
    Shepson, P.B., T.E. Kleindienst, E.O. Edney, G.R. Namie, J.H. Pittman, L.T. Cupitt, and L.D. Claxton (1985) The mutagenic activity of irradiated toluene/NOx /H2O/air mixtures. Env. Sci. Tech. 19:249–255.CrossRefGoogle Scholar
  17. 17.
    Shepson, P.B., T.E. Kleindienst, CM. Nero, D.N. Hodges, L.T. Cupitt, and L.D. Claxton (1987) Allyl chloride: The mutagenic activity of its photooxidation products. Env. Sci. Tech. 21:568–573.CrossRefGoogle Scholar
  18. 18.
    Shepson, P.B., T.E. Kleindienst, E.O. Edney, L.T. Cupitt, and L.D. Claxton (1987) The production in the atmosphere of mutagenic products from simple hydrdocarbons. In Short-term Bioassays in the Analysis of Complex Environmental Mixtures, V, S.S. Sandhu, D.M. DeMarini, M.J. Mass, M.M. Moore, and J.L. Mumford, eds. Plenum Publishing Corp., New York, pp. 277–290.Google Scholar
  19. 19.
    Singh, H.B., L.J. Salas, A. Smith, R. Stiles, and H. Shigeishi (1981) Atmospheric Measurements of Selected Hazardous Organic Chemicals. U.S. Environmental Protection Agency, Report Number EPA-600/3–81–032.Google Scholar
  20. 20.
    Tice, R.R., D.L. Costa, and K.M. Schaich (1982) Genotoxic Effects of Airborne Agents, Plenum Publishing Corporation, New York, NY.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Larry D. Claxton
    • 1
  • T. E. Kleindienst
    • 2
  • Erica Perry
    • 3
  • Larry T. Cupitt
    • 4
  1. 1.Health Effects Research LaboratoryU.S. Environmental Protection AgencyResearch Triangle ParkUSA
  2. 2.Northrop Services InternationalResearch Triangle ParkUSA
  3. 3.Environmental Health Research and Testing, Inc.Research Triangle ParkUSA
  4. 4.Atmospheric Research and Exposure Assessment LaboratoryU.S. Environmental Protection AgencyResearch Triangle ParkUSA

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