32P-Postlabeling DNA Adduct Assay: Cigarette Smoke-Induced DNA Adducts in the Respiratory and Nonrespiratory Rat Tissues

  • R. C. Gupta
  • C. G. Gairola
Part of the Environmental Science Research book series (ESRH, volume 39)


Several epidemiological studies have strongly implicated cigarette smoking with higher incidence of respiratory tract cancer including those of larynx, the oral cavity, and the lung (4). In addition, smokers face an increased risk for cancer of the pancreas and bladder, among other organs (14,20).


Cigarette Smoke Adduct Level Mainstream Smoke Total Particulate Matter Aryl Hydrocarbon Hydroxylase 


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  1. 1.
    Binns, R. (1977) Inhalation toxicity studies on cigarette smoke IV. Expression of the dose of smoke particulate material to the lungs of experimental animals. Toxicology 7:189–195.PubMedCrossRefGoogle Scholar
  2. 2.
    Dalbey, W.E., P. Nettesheim, R. Griesemer, J.E. Caton, and M.R. Guerin (1980) Chronic inhalation of cigarette smoke by F344 rats. JNCI 64:383–390.PubMedGoogle Scholar
  3. 3.
    Dontenwill, W.P. (1974) Tumorigenic effect of chronic cigarette smoke inhalation on Syrian golden hamster. In Experimental Lung Cancer. Carcinogenesis and Bioassays, E. Karbe and E. Park, eds. Springer-Verlag, New York, pp. 331–368.Google Scholar
  4. 4.
    Fielding, J.E. (1985) Smoking—Health effects and control, Part II. New Eng. J. Med. 313:555–561.PubMedCrossRefGoogle Scholar
  5. 5.
    Gairola, C. (1987) Pulmonary aryl hydrocarbon hydroxylase activity of mice, rats, and guinea pigs following long-term exposure to mainstream and sidestream cigarette smoke. Toxicology 45:177–184.PubMedCrossRefGoogle Scholar
  6. 6.
    Garg, A., and R.C. Gupta (1988) Tissue-specific DNA modifications in untreated, aged rats as detected by 32P-adduct assay. Proc. Am. Assoc. Cancer Res. 29:104.Google Scholar
  7. 7.
    Griffith, R.B., and R. Handcock (1985) Simultaneous mainstream-sidestream smoke exposure systems I. Equipment and procedures. Toxicology 34:123–138.PubMedCrossRefGoogle Scholar
  8. 8.
    Griffith, R.B., and S. Standafer (1985) Simultaneous mainstream-sidestream smoke exposure systems II. The rat exposure system. Toxicology 35:13–24.PubMedCrossRefGoogle Scholar
  9. 9.
    Gupta, R.C. (1984) Nonrandom binding of the carcinogen N-hydroxy-2-acetylaminofluorene to repetitive sequences of rat liver DNA in vivo. Proc. Natl. Acad. Sci., USA 81:6943–6947.PubMedCrossRefGoogle Scholar
  10. 10.
    Gupta, R.C. (1985) Enhanced sensitivity of 32P-postlabeling analysis of aromatic carcinogen-DNA adducts. Cancer Res. 45:5656–5662.PubMedGoogle Scholar
  11. 11.
    Gupta, R.C., M.V. Reddy, and K. Randerath (1982) 32P-Postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts. Carcinogenesis (Lond.) 3:1081–1092.CrossRefGoogle Scholar
  12. 12.
    Gupta, R.C., M.L. Sopori, and C.G. Gairola (1989) Formation of cigarette smoke-induced DNA adducts in the rat lung and nasal mucosa. Cancer Res. 49:1916–1920.PubMedGoogle Scholar
  13. 13.
    Gupta, R.C., K. Earley, J. Locker, and B. Lombardi (1987) 32P-Postlabeling analysis of liver DNA adducts in rats chronically fed a choline-devoid diet. Carcinogenesis (Lond.) 8:187–189.CrossRefGoogle Scholar
  14. 14.
    International Agency for Research on Cancer Tobacco Smoking (1986) IARC Monograph 38:199–293.Google Scholar
  15. 15.
    Martin, G.M., A.C. Smith, D.J. Ketterer, C.E. Ogburn, and C.M. Disteche (1985) Increased chromosomal aberrations in first meta-phases of cells isolated from the kidneys of aged mice. Israel J. Med. Sci. 21:296–301.PubMedGoogle Scholar
  16. 16.
    Randerath, K., M.V. Reddy, and R.M. Disher (1986) Age- and tissue-related DNA modifications in untreated rats: Detection by 32P-postlabeling assay and possible significance for spontaneous tumor induction and aging. Carcinogenesis (Lond.) 7:1615–1617.CrossRefGoogle Scholar
  17. 17.
    Reddy, M.V., and K. Randerath (1986) Nuclease P1-mediated enhancement of sensitivity of 32P-postlabeling test for structurally diverse DNA adducts. Carcinogenesis (Lond.) 7:1543–1551.CrossRefGoogle Scholar
  18. 18.
    Schneider, E.L. (1985) Cytogenetics of aging. In Handbook of the Biology of Aging, C.E. Finch and E.L. Schnieder, eds. Van Nostrand Reinhold, New York, pp. 357–373.Google Scholar
  19. 19.
    Scmookler-Reiss, R.J., and S. Goldstein (1985) Genetic diversification and the clonality of senscence. In Review of Biological Research in Aging, Vol. 2, M. Rothstein, ed. Alan R. Liss, New York, pp. 115–139.Google Scholar
  20. 20.
    U.S. Surgeon General (1982) The Health Consequences of Smoking—Cancer (PHS) 82–50179:5–8.Google Scholar
  21. 21.
    Wehner, A.P., G.E. Dagle, E.M. Milliman, P.W. Phelps, D.B. Carr, J.R. Decker, and R.E. Filpy (1981) Inhalation bioassays of cigarette smoke in rats. Tox. Appl. Pharm. 61:1–17.CrossRefGoogle Scholar
  22. 22.
    Wynder, E.L., and D. Hoffman (1967) Tobacco and Tobacco Smoke-studies in Experimental Carcinogenesis. Academic Press, New York, pp. 730–752.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • R. C. Gupta
    • 1
    • 3
  • C. G. Gairola
    • 2
    • 3
  1. 1.Department of Preventive Medicine and Environmental HealthUniversity of KentuckyLexingtonUSA
  2. 2.Tobacco and Health Research InstituteUniversity of KentuckyLexingtonUSA
  3. 3.Graduate Center for ToxicologyUniversity of KentuckyLexingtonUSA

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