Heterogeneous Distribution of L-DOPA Immunoreactivity in Dopaminergic Neurons of the Rat Midbrain
Behavioral and biochemical studies have supported the idea that the dopaminergic projection systems from the midbrain to the basal ganglia or to the limbic cortex could be a key role in motor or psychomotor functions.1,2 Midbrain dopamine (DA) neurons were first histochemically identified by the Falck-Hillarp formaldehyde histofluorescence technique,3,4 and later further analyzed by immunocytochemistry using antibodies against tyrosine hydroxylase (TH).5 Recent advances in immunological methodology has enabled the production of antibodies against cathecholamines themselves.6,7,8 Owing to these antisera, we could analyze the morphological status of DA or its precursor, L-DOPA, at the single cell level by immunocytochemical means.8,9,10 In the present study, we report the localization characteristics of L-DOPA in midbrain DA neurons of the rat brain defined by specific and sensitive antiserum raised against L-DOPA, and the subdivision of the dopaminergic neuronal cell group by L-DOPA immunostaining.
KeywordsTyrosine Hydroxylase Basal Forebrain Limbic Cortex Sodium Metabisulfite Catecholamine Neuron
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- 4.A. Dahlstrom, and K. Fuxe, Evidence for the existence of monoamine-containing neurons in the central nervous system. I. Demonstration of monoamines in the cell bodies of brain stem neurons, Acta Physiol.Scand. 62 (Suppl. 232): 1 (1964).Google Scholar
- 5.T. Hökfelt, R. Martensson, A. Björklund, S. Kleinan, and M. Goldstein, Distributional maps of tyrosine-hydroxylaseimmunoreactive neurons in the rat brain, in “Handbook of Chemical Neuroanatomy, Vol.2”, A. Björklund and T. Hökfelt, ed., Elsevier, Amsterdam (1984).Google Scholar
- 13.L. A. Sternberger, “Immunocytochemistry”, Wiley, New York (1986).Google Scholar
- 14.A. Björklund, and O. Lindvall, Dopamine-cortaining systems in the CNS, in: “Handbook of Chemical Neuroanatomy, Vol. 2”, A. Björklund and T.Hòkfelt, eds., Elsevier, Amsterdam (1984).Google Scholar