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A Study on Postmortem Changes in Vasopressin mRNA in Rat Brain Using a Non-Isotopic Method

  • Iwahide Noguchí
  • Heii Arai
  • Takasi Moroji
  • Reiji Iizuka
Part of the Advances in Behavioral Biology book series (ABBI, volume 38A)

Abstract

Recent studies in Alzheimer type dementia(ATD) disclosed a depletion of several neuropeptides in postmortem brains. With the progress of molecular biology, we can also study changes in neuropeptides messenger RNA(mRNA) to interpret the depletion in ATD brains. In general, radioactive probe has been used in the hybridization technique. However, we already reported in situ hybridization histochemistry (ISHH) method using a biotinylated oligonucleotide probe(Arai et al.,1988). In the present study, we tried to establish a method of dot and northern blot analysis using nonradioactive probes for quantification of mRNA.

Keywords

Northern Blot Analysis Postmortem Brain Postmortem Change Radioactive Probe Postmortem Delay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Iwahide Noguchí
    • 1
  • Heii Arai
    • 1
  • Takasi Moroji
    • 2
  • Reiji Iizuka
    • 1
  1. 1.Department of PsychiatryJuntendo University School of MedicineTokyoJapan
  2. 2.Depertment of PsychopharmacologyPsychiatric Research Institute of TokyoTokyoJapan

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