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Differential Expression of Three Types of Amyloid Protein Precursor mRNA in Alzheimer’s Disease Brain

  • Seigo Tanaka
  • Yasuyuki Takahashi
  • Satoshi Shiojiri
  • Nobuya Kitaguchi
  • Hirataka Ito
  • Kunihiro Ueda
  • Shigenobu Nakamura
Part of the Advances in Behavioral Biology book series (ABBI, volume 38A)

Abstract

Deposition of amyloid β-protein in senile plaque cores and cerebral vessels is one of the characteristic findings in Alzheimer’s disease brain. Complementary DNA (cDNA) encoding the precursor of amyloid β-protein (APP695) was cloned and sequenced1. The precursor has structural features characteristic of cell surface receptors. We cloned APP cDNAs from a cDNA library of a human glioblastoma cell line, and found two more cDNAs encoding APPs (APP751 and APP770) together with APP695 cDNA previously reported2. APP751 has a 56 amino-acid insert, and APP770 has an additional 19 amino-acid insert (Fig. 1). These two inserts are encoded by exons, 7 and 8 2,3, respectively. APP gene exists as a single copy, indicating that the three types of APP mRNA are produced by alternative splicing from a common transcript. The amino-acid sequence encoded by exon 7 is highly homologous to the basic trypsin inhibitor family. The fact that this APP fragment has actually a protease inhibitor activity was shown by an in vitro expression experimeht2.

Keywords

Human Glioblastoma Cell Line Common Transcript Amyloid Protein Precursor mRNA Protease Inhibitor Domain pSP64 Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Seigo Tanaka
    • 1
  • Yasuyuki Takahashi
    • 3
  • Satoshi Shiojiri
    • 3
  • Nobuya Kitaguchi
    • 3
  • Hirataka Ito
    • 3
  • Kunihiro Ueda
    • 2
  • Shigenobu Nakamura
    • 1
  1. 1.Department of NeurologyFaculty of Medicine, Kyoto UniversityKyotoJapan
  2. 2.Department of Clinical Science and Laboratory Medicine, Faculty of MedicineKyoto UniversityKyotoJapan
  3. 3.Bio-Science LaboratoryLife Science Research Laboratories Asahi Chemical Industry Co. Ltd.ShizuokaJapan

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