Assay System for Neurotoxicants Causing Parkinson’s Disease: 1-Methyl-4-Phenylpyridinium Ion (MPP+) Inhibits Survival of Cultured Neurons from Substantia Nigra of Fetal Monkey(Macaca fascicularis)
Most neurotoxicological research is carried out using living rodents (mice and rats). This has three main drawbacks. The first is the fundamental difficulty of extrapolating these results to human toxicity. However, obtaining statistical data on living non-human primates is practically impossible because of its high cost. The second is the killing of a great number of living animals in opposition to recent animal protection movements. The third is the difficulty of molecular and cellular studies in vivo. To avoid or decrease these scientific and social problems, it is worthwhile exploring the possibility of using in vitro preparations such as brain slices1 or cultured neurons2.
KeywordsTyrosine Hydroxylase Substantia Nigra Dopaminergic Neuron Microtubule Associate Protein Human Toxicity
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