Effect of MPP+ and TIQ on Cultured Rat Midbrain Dopaminergic Neurons
MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induces Parkinson’s disease in humans, monkeys and some animals (1,2). MPTP was oxidized to 1-methyl-4-phenylpyrdinium ion (MPP+) by monoamine oxidase (3). Nagatsu and Hirata reported that endogenous candidate of pyridinium compound, tetrahydroisoquinoline (TIQ) decreased tyrosine hydroxylase (TH) and 3,4-dihydroxyphenylacetic acid in mice striatum (4). In the current investigation, effect of MPP+ and TIQ on cultured rat midbrain dopaminergic neurons were studied, using cell or organotypic culture systems.
KeywordsTyrosine Hydroxylase Positive Neuron Organotypic Culture Ventral Midbrain Mouse Striatum
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