Advertisement

Dopamine Immunocytochemistry in the Nigrostriatal System of Pre- and Postnatal Mice

  • Ikuko Nagatsu
  • Masao Sakai
  • Kiyokuni Miura
  • Kazuyoshi Watanabe
Part of the Advances in Behavioral Biology book series (ABBI, volume 38A)

Abstract

Brain tissue transplantation is now used for the functional recovery of experimental parkinsonian animals and parkinsonian patients. For example, grafts of catecholamine(CA)-containing neuronal cells, such as those in fetal brain tissues from the substantia nigra(SN) and ventral tegmental area(VTA), were able to abolish the abnormal rotating movement in animal models of hemiparkinsonism`. Tyrosine hydroxylase-like immunoreactivity (TH-LI) in the SN and VTA of fetal brain has been generally used to assess the functional activity of the grafts. However, even though TH-LI is demonstrated in the brain tissues used for grafting, aromatic L-amino acid decarboxylase(AADC)and dopamine(DA)-LI should also be proved to be present. In order to confirm at what stages of development the SN and VTA tissues are most appropriate for brain grafting, we obtained mouse brain taken during prenatal and postnatal development and examined the neurons containing CA-synthesizing enzymes immunocytochemically using specific antisera to TH, AADC, and DA2.

Keywords

Substantia Nigra Ventral Tegmental Area Kainic Acid Medial Forebrain Bundle Postnatal Development 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Björklund, S.B. Dunnett, U. Stenevi, M.E. Lewis, and S.D.Iversen, Reinnervation of the denervated striatum by substantia nigra transplants: functional consequences as revealed by pharmacological and sensorimotor testing, Brain Res 199: 307-333 (1980).Google Scholar
  2. 2.
    I. Nagatsu, M. Sakai, M. Yoshida, and T. Nagatsu, Aromatic L-amino acid decarboxylase-immunoreactive neurons in and around the cerebrospinal fluid-contacting neurons of the central canal do not contain dopamine or serotonin in the mouse and rat spinal cord, Brain Res 475: 91 - 102 (1988).PubMedCrossRefGoogle Scholar
  3. 3.
    I. Nagatsu, S. Furune, H. Ichinose, M. Spatz, and T. Nagatsu, Quantitative analysis of reduction of aromatic L-amino acid decarboxylase-and tyrosine hydroxylase-like immunoreactivities in the nigrostriatal dopaminergic neurons of MPTP-treated mice, Biogenic Amines 6: 263-277 (1989).Google Scholar
  4. 4.
    S. Furune, K. Miura, K. Watanabe, S. Nagao, H. Takahashi, M. Sakai, M..Spatz, and I. Nagatsu, Transplacental effect of 1-methyl-4-phenyl-1,2,3,6tetrahydropyridine(MPTP) on brain dopaminergic neurons in the mouse. An immunohistochemical study, Acta Neuropathologica, in press.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Ikuko Nagatsu
    • 1
  • Masao Sakai
    • 1
  • Kiyokuni Miura
    • 2
  • Kazuyoshi Watanabe
    • 2
  1. 1.Dept. of AnatSchool of Med.,Fujita Health UnivToyoake AichiJapan
  2. 2.Dept. of PediatricsNagoya Univ. School of MedNagoya 464Japan

Personalised recommendations