Dopamine Immunocytochemistry in the Nigrostriatal System of Pre- and Postnatal Mice
Brain tissue transplantation is now used for the functional recovery of experimental parkinsonian animals and parkinsonian patients. For example, grafts of catecholamine(CA)-containing neuronal cells, such as those in fetal brain tissues from the substantia nigra(SN) and ventral tegmental area(VTA), were able to abolish the abnormal rotating movement in animal models of hemiparkinsonism`. Tyrosine hydroxylase-like immunoreactivity (TH-LI) in the SN and VTA of fetal brain has been generally used to assess the functional activity of the grafts. However, even though TH-LI is demonstrated in the brain tissues used for grafting, aromatic L-amino acid decarboxylase(AADC)and dopamine(DA)-LI should also be proved to be present. In order to confirm at what stages of development the SN and VTA tissues are most appropriate for brain grafting, we obtained mouse brain taken during prenatal and postnatal development and examined the neurons containing CA-synthesizing enzymes immunocytochemically using specific antisera to TH, AADC, and DA2.
KeywordsSubstantia Nigra Ventral Tegmental Area Kainic Acid Medial Forebrain Bundle Postnatal Development
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