Characterization of Alzheimer Neurofibrillary Tangle (NFT) Epitopes and the Binding Site for the Regulatory Subunit (RII) of cAMP-Dependent Protein Kinase II in Human Microtubule-Associated Protein 2 (MAP-2)
Monoclonal antibodies (mAbs) generated against enriched protein preparations of Alzheimer neurofibrillary tangles (NFT) have been used as probes to study the structure of the proteins associated with NFT1. Anti-NFT antibodies have been found to cross react with purified neurofilaments, ubiquitin, tau and MAP-2 suggesting that NFT represent a derangement of the neuronal cytoskeleton. The question remains, however, whether these proteins are integral components of NFT or have regions of homology with NFT proteins. To address this question we screened a human brain cDNA expression library with a pool of mAbs raised against SDS-extracted NFT and have isolated several cDNA clones2. One of these clones detects 6 kb and 9.5 kb transcripts in human neuroblastoma mRNA preparations2. Sequence analysis of the cDNA has determined that the 1.7 kb insert encodes 569 amino acids of the projection arm of MAP-23.
KeywordsRegulatory Subunit Progressive Supranuclear Palsy Paired Helical Filament Amino Acid Region Paired Helical Filament
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