Diagnosis of Cerebral Amyloid Angiopathy with Cerebral Hemorrhage by Enzyme-Linked Immunosorbent Assay (ELISA) of Cystatin C in Cerebrospinal Fluid
A lower level of cystatin C in cerebrospinal fluid (CSF) is one of the useful diagnostic markers of hereditary cerebral hemorrhage with amyloidosis in Iceland. Therefore we set up an assay to determine the level of cystatin C in CSF for diagnosis of cerebral amyloid angiopathy (CAA) associated with cerebral hemorrhage and cystatin C. We carried out an sandwich enzyme immunosorbent assay using monoclonal mouse anti-cystatin C and polyclonal rabbit anti-cystatin C antibodies. We examined CSF from nine cases of cerebral hemorrhage and fifty reference cases of other neurological diseases. Four patients with cerebral hemorrhage showed a low level of cystatin C and clinical manifestations suggestive of CAA. Our study showed that ELISA is useful for the diagnosis of CAA associated with cerebral hemorrhage and cystatin C.
KeywordsCerebral Hemorrhage Cerebral Amyloid Angiopathy Cysteine Proteinase Inhibitor Thalamic Hemorrhage Immunoreactive Substance
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- 4.S. Fujihara, K. Shimode, S. Kobayashi, and T. Tsunematsu, Possibly “familial” cerebral angiopathy in Japan: Immunohistochemical identification of gamma-trace, in: “Amyloid and Amyloidosis,” T. Isobe, S. Araki, F. Uchino, S. Kito, and E. Tsubura, eds., Plenum Publishing Co., New. York (1988).Google Scholar
- 5.K. Maruyama, S. Ikeda, T. Ishihara, D. Allsop, and N. Yanagisawa, Immunohistochemical characterization of cerebrovascular amyloid in 46 cases using antibodies to ß-protein and cystatin C, Stroke, in press.Google Scholar
- 6.H. Löfberg, A. O. Grubb, E. K. Nilsson, O. Jensson, G. Gudmundsson, H. Blondal, A. Arnason, and L. Thorsteinsson, Immunohistochemical characterization of the amyloid deposits and quantitation of pertinent cerebrospinal fluid proteins in hereditary cerebral hemorrhage with amyloidosis, Stroke 18: 431 (1987)Google Scholar