Diagnosis of Cerebral Amyloid Angiopathy with Cerebral Hemorrhage by Enzyme-Linked Immunosorbent Assay (ELISA) of Cystatin C in Cerebrospinal Fluid

  • Koichi Shimode
  • Shigeyoshi Fujihara
  • Morihiko Nakamura
  • Shotai Kobayashi
  • Tokugoro Tsunematsu
Part of the Advances in Behavioral Biology book series (ABBI, volume 38A)

Summary

A lower level of cystatin C in cerebrospinal fluid (CSF) is one of the useful diagnostic markers of hereditary cerebral hemorrhage with amyloidosis in Iceland. Therefore we set up an assay to determine the level of cystatin C in CSF for diagnosis of cerebral amyloid angiopathy (CAA) associated with cerebral hemorrhage and cystatin C. We carried out an sandwich enzyme immunosorbent assay using monoclonal mouse anti-cystatin C and polyclonal rabbit anti-cystatin C antibodies. We examined CSF from nine cases of cerebral hemorrhage and fifty reference cases of other neurological diseases. Four patients with cerebral hemorrhage showed a low level of cystatin C and clinical manifestations suggestive of CAA. Our study showed that ELISA is useful for the diagnosis of CAA associated with cerebral hemorrhage and cystatin C.

Keywords

Cerebral Hemorrhage Cerebral Amyloid Angiopathy Cysteine Proteinase Inhibitor Thalamic Hemorrhage Immunoreactive Substance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Koichi Shimode
    • 1
  • Shigeyoshi Fujihara
    • 1
  • Morihiko Nakamura
    • 1
  • Shotai Kobayashi
    • 1
  • Tokugoro Tsunematsu
    • 1
  1. 1.Third Division of Internal MedicineShimane Medical UniversityIzumo 693Japan

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