Diagnosis of Cerebral Amyloid Angiopathy with Cerebral Hemorrhage by Enzyme-Linked Immunosorbent Assay (ELISA) of Cystatin C in Cerebrospinal Fluid
A lower level of cystatin C in cerebrospinal fluid (CSF) is one of the useful diagnostic markers of hereditary cerebral hemorrhage with amyloidosis in Iceland. Therefore we set up an assay to determine the level of cystatin C in CSF for diagnosis of cerebral amyloid angiopathy (CAA) associated with cerebral hemorrhage and cystatin C. We carried out an sandwich enzyme immunosorbent assay using monoclonal mouse anti-cystatin C and polyclonal rabbit anti-cystatin C antibodies. We examined CSF from nine cases of cerebral hemorrhage and fifty reference cases of other neurological diseases. Four patients with cerebral hemorrhage showed a low level of cystatin C and clinical manifestations suggestive of CAA. Our study showed that ELISA is useful for the diagnosis of CAA associated with cerebral hemorrhage and cystatin C.
KeywordsAlbumin Cysteine Trypsin Fibril Spectrophotometry
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