Interleukin-3 Promotes Neurite Outgrowth and Elevates Choline Acetyltransferase Activity
Alzheimer’s disease (AD) is associated with the degeneration of cholinergic neurons in the hippocampus and basal forebrain. Since nerve growth factor (NGF) was found to enhance choline acetyltransferase (ChAT) activity, and the infusion of NGF into the rat cerebral ventricle prevented death of lesioned septal cholinergic neurons and improved cognitive functions in old animals, NGF has been proposed as a therapeutic agent for AD. As basic fibroblast growth factor has a similar effect in vivo, we looked for other trophic factors for central cholinergic neurons. In this study we demonstrate that interleukin-3 (IL-3), which plays a role in the growth and differentiation of hematopoietic lineage cells, also acts as a novel trophic factor for central cholinergic neurons in primary cultures of mouse septal neurons and in cholinergic hybridoma line cell SN220.127.116.11.
KeywordsNerve Growth Factor Glial Fibrillary Acidic Protein Cholinergic Neuron Trophic Factor ChAT Activity
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