Co-Localization of Cholinergic and GABAergic Traits in in Vitro Septohippocampal Neurons from Developing Rats
It is well known that basal forebrain neurons are involved in Alzheimer’s disease. Massive neuronal loss occurs in the basal forebrain as well as in the cortical areas that are interconnected with this region. In rodents, nerve growth factor (NGF) acts on cholinergic neurons in the basal forebrain to induce choline acetyltransferase activity.1 It was suggested that survival of the basal forebrain neurons is also dependent on NGF during development2 and in adult life.3 We have offered direct in vitro evidence that NGF in fact promotes the survival of hippocampus-projecting neurons in the medial septum and vertical limb of the diagonal band of developing rats.4 The septohippocampal neurons were identified by retrograde-labeling with fluorescent latex microspheres that had been injected into the hippocampus in vivo. The number of microsphere-labeled projection neurons in the cultures supplemented with 100 ng/ml NGF was much greater than that without NGF.
KeywordsNerve Growth Factor AChE Activity Basal Forebrain Medial Septum Nerve Growth Factor Receptor
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- 6.Y. Arimatsu and M. Miyamoto (submitted).Google Scholar