Expression of Nicotinic Acetylcholine Receptor mRNA in the Rat Cerebral Cortex after Lesioning of the Nucleus Basalis Magnocellularis
We investigated the effect of a unilateral lesion made in the nucleus basalis magnocellularis (nbm) on the expression of nicotinic acetylcholine receptors (nAChR’s) in the rat cerebral cortex. Cortical [3H]nicotine binding was not affected by the nbm lesion. Expression of nAChR mRNA in the cerebral cortex was determined by use of cDNA clones coding for nAChR subunits alpha-3, alpha-4, and beta-2. At 1 week after the lesioning, expression of alpha-4 and beta-2 was increased by 82% and 19%, respectively. By 4 weeks afterward, expression levels of these nAChR subunits on the ipsilateral side did not differ from those on the control side. Expression of alpha-3 was not altered. These results suggest that nAChR transcripts are regulated by cell-to-cell interactions and may represent supporting evidence for the occurrence of supersensitivity in deafferentated cholinergic neurons.
KeywordsNicotinic Acetylcholine Receptor Control Side ChAT Activity Ibotenic Acid nAChR Subunit
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