Effects of Redox State for Ubiquinone-10 on the Membrane Fluidity of Cardiolipin-Containing Liposomes
The effect of redox state for ubiquinone-10 on the membrane fluidity of cardiolipin-containing liposomes has been studied with spin labels 5-and 16-doxyl stearate derivatives. Incorporation of ubiquinone-10 or its reduced form to the membrane significantly increases the membrane fluidity at the depth near membrane surface. The reduced form has more significant effect than oxidized form has. In the core of lipid bilayer, however, rotational correlation time of spin label 16-NS increases upon incorporation of ubiquinone-10 or its reduced form. After binding of adriamycin to cardiolipin, the fluidizing effect of ubiquinone-10 is abolished. It is suggested that cardiolipin plays important role in determination of dynamic characteristics of the membrane via its interaction with ubiquinone-10.
KeywordsElectron Spin Resonance Lipid Bilayer Electron Spin Resonance Spectrum Membrane Fluidity Reduced Form
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