Advertisement

Bioenergetics pp 353-362 | Cite as

Nuclear Genes Encoding Two Subunits of Human Mitochondrial Cytochrome bc1 Complex, Cytochrome c1 and Ubiquinone-Binding Protein: Their Structural Organization of the 5′-Flanking Regions and Chromosomal Localization

  • Hiroshi Suzuki
  • Yoshitaka Hosokawa
  • Haruo Toda
  • Morimitsu Nishikimi
  • Akio Matsukage
  • Michihiro C. Yoshida
  • Takayuki Ozawa

Summary

We have isolated nuclear genes encoding human cytochrome c1 (C1) and ubiquinone-binding protein (QP) of cytochrome bc1 complex. The C1 and QP genes span 2.4 and 4.5 kilobase pairs, respectively. All intron/exon splice junctions in both genes follow the GT/AG rule. The 5′-flanking region of the C1 gene contains seven putative GC boxes as typical transcriptional regulatory sequence elements but lacks TATA and CCAAT boxes. The same region of the QP gene contains four putative CCAAT boxes and one putative NF-Y binding site but lacks TATA and GC boxes. In the 5′-flanking regions of both genes, there are homologous sequences to AP-1 recognition site and three common sequences, 5′-TATTCAGGT-3′, 5′-ATCTGGCT-3′, and 5′-TGGTGA(T/G)AG-3′. A homologous sequence to each of the three common sequences is also found in the 5′-flanking regions of the genes for the β subunit of human F0F1-ATPase and rat somatic cytochrome c. It may be that the common sequences play an important role in the coordinate expression of nuclear genes encoding mitochondrial proteins responsible for oxidative phosphorylation. Southern blot analyses showed that both genes are present in a single copy in the human genome and are located on chromosome 8.

Keywords

Flank Region Nuclear Gene Sense Strand Antisense Strand Common Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Battini, R., Ferrari, S., Kaczmarek, L., Calabretta, B., Chen, S-T., and Baserga, R. (1987) J. Biol. Chem. 262, 4355–4359.PubMedGoogle Scholar
  2. 2.
    Kaczmarek, L., Calabretta, B., and Baserga, R. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 5375–5379.PubMedCrossRefGoogle Scholar
  3. 3.
    Glaichenhaus, N., Leopold, P., and Cuzin, F. (1986) EMBO J. 5, 1261–1265.PubMedGoogle Scholar
  4. 4.
    Liu, H.-T., Baserga, R., and Mercer, W. E. (1985) Mol. Cell. Biol. 5, 2936–2942.PubMedGoogle Scholar
  5. 5.
    Yamamoto, A., Horai, S., and Yuasa, Y. (1989) Biochem. Biophys. Res. Commun. 159, 1100–1106.PubMedCrossRefGoogle Scholar
  6. 6.
    Ohta, S., Goto, K., Arai, H., and Kagawa, Y. (1987) FEBS Lett. 226, 171–175.PubMedCrossRefGoogle Scholar
  7. 7.
    Tanaka, M., Miyabayashi, S., Nishikimi, M., Suzuki, H., Shimomura, Y., Ito, K., Narisawa, K., Tada, K., and Ozawa, T. (1988) Pediatric Res. 24, 447–454.CrossRefGoogle Scholar
  8. 8.
    Ozawa, T., Yoneda, M., Tanaka, M., Ohno, K., Sato, W., Suzuki, H., Nishikimi, M., Yamamoto, M., Nonaka, I., and Horai, S. (1988) Biochem. Biophys. Res. Commun. 154, 1240–1247.PubMedCrossRefGoogle Scholar
  9. 9.
    Zeviani, M., Servidei, S., Gellera, C., Bertini, E., DiMauro, S., and DiDonato, S. (1989) Nature 339,309–311.PubMedCrossRefGoogle Scholar
  10. 10.
    Suzuki, H., Hosokawa, Y., Nishikimi, M., and Ozawa, T. (1989) J. Biol. Chem. 264, 1368–1374.PubMedGoogle Scholar
  11. 11.
    Ohta, S., Tomura, H., Matsuda, K., and Kagawa, Y. (1988) J. Biol. Chem. 263, 11257–11262.PubMedGoogle Scholar
  12. 12.
    Maguire, D. J., Day, A. R., Borthwick, I. A., Srivastava, G., Wigley, P. L., May, B. K., and Elliott, W. H. (1986) Nucleic Acids Res. 14, 1379–1391.PubMedCrossRefGoogle Scholar
  13. 13.
    Dorsman, J. C., van Heeswijk, W. C., and Grivell, L. A. (1988) Nucleic Acids Res. 16, 7287–7301.PubMedCrossRefGoogle Scholar
  14. 14.
    Suzuki, H., Hosokawa, Y., Toda, H., Nishikimi, M., and Ozawa, T. (1989) Biochem. Biophys. Res. commun. 161, 371–378.PubMedCrossRefGoogle Scholar
  15. 15.
    Semba, K., Yamanashi, Y. Nishizawa, M., Sukegawa, J., Yoshida, M., Sasaki, M., Yamamoto, T., and Toyoshima, K. (1985) Science 227, 1038–1040.PubMedCrossRefGoogle Scholar
  16. 16.
    Nishikimi, M., Ohta, S., Suzuki, H., Tanaka, T., Kikkawa, F., Tanaka, M., Kagawa, Y., and Ozawa, T. (1988) Nucleic Acids Res. 16, 3577.PubMedCrossRefGoogle Scholar
  17. 17.
    Suzuki, H., Hosokawa, Y., Toda, H., Nishikimi, M., and Ozawa, T.(1988) Biochem. Biophys. Res. Commun. 156, 987–994.PubMedCrossRefGoogle Scholar
  18. 18.
    Arndt, K., and Fink, G. R. (1986) Froc. Natl. Acad. Sci. U. S. A. 83, 8516–8520.CrossRefGoogle Scholar
  19. 19.
    Bohmann, D., Bos, T. J., Admon, A., Nishimura, T., Vogt, P. K., and Tjian, R. (1987) Science 238, 1386–1392.PubMedCrossRefGoogle Scholar
  20. 20.
    Kadonaga, J. T., Jones, K. A., and Tijan. R. (1986) Trends Biochem. Sci. 11, 20–23.CrossRefGoogle Scholar
  21. 21.
    Evans, M. J., and Scarpulla, R. C. (1988) Mol. Cell. Biol. 8, 35–41.PubMedGoogle Scholar
  22. 22.
    Nishikimi, M., Suzuki, H., Yamaguchi, M., Matsukage, A., Yoshida, M.C., and Ozawa, T. (1988) Biochem. Intl. 16, 655–660.Google Scholar
  23. 23.
    Matsukage, A., Yamaguchi, M., Utsumi, K. R., Hayashi, Y., Ueda, R., and Yoshida, M. C. (1986) Jpn. J. Cancer Res. (Gann) 77, 330–333.Google Scholar
  24. 24.
    Robin, E. D., and Wong, R. (1988) J. Cell. Physiol. 136, 507–513.PubMedCrossRefGoogle Scholar
  25. 25.
    Kingston, R. E., Baldwin, A. S., and Sharp, P. A. (1985) Cell 41, 3–5.PubMedCrossRefGoogle Scholar
  26. 26.
    Mutvei, A., Kuzela, S., and Nelson, B. D. (1989) Eur. J. Biochem.180, 235–240.PubMedCrossRefGoogle Scholar
  27. 27.
    Williams, R. S. (1986) J. Biol. Chem. 261, 12390–12394.PubMedGoogle Scholar
  28. 28.
    Williams, R. S., Garcia-Moll, M., Mellor, J., Salmons, S., and Harlan, W. (1987) J. Biol. Chem. 262, 2764–2767.PubMedGoogle Scholar
  29. 29.
    Hood, D. A., Zak, R., and Pette, D. (1989) Eur. J. Biochem. 179, 275–280.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Hiroshi Suzuki
    • 1
  • Yoshitaka Hosokawa
    • 1
  • Haruo Toda
    • 1
  • Morimitsu Nishikimi
    • 1
  • Akio Matsukage
    • 2
  • Michihiro C. Yoshida
    • 3
  • Takayuki Ozawa
    • 1
  1. 1.Department of Biomedical Chemistry, Faculty of MedicineUniversity of NagoyaNagoya 466Japan
  2. 2.Laboratory of Cell BiologyAichi Cancer Center Research InstituteNagoya 464Japan
  3. 3.Chromosome Research Unit, Faculty of SciencesHokkaido UniversitySapporo 060Japan

Personalised recommendations