Nuclear Genes Encoding Two Subunits of Human Mitochondrial Cytochrome bc1 Complex, Cytochrome c1 and Ubiquinone-Binding Protein: Their Structural Organization of the 5′-Flanking Regions and Chromosomal Localization
We have isolated nuclear genes encoding human cytochrome c1 (C1) and ubiquinone-binding protein (QP) of cytochrome bc1 complex. The C1 and QP genes span 2.4 and 4.5 kilobase pairs, respectively. All intron/exon splice junctions in both genes follow the GT/AG rule. The 5′-flanking region of the C1 gene contains seven putative GC boxes as typical transcriptional regulatory sequence elements but lacks TATA and CCAAT boxes. The same region of the QP gene contains four putative CCAAT boxes and one putative NF-Y binding site but lacks TATA and GC boxes. In the 5′-flanking regions of both genes, there are homologous sequences to AP-1 recognition site and three common sequences, 5′-TATTCAGGT-3′, 5′-ATCTGGCT-3′, and 5′-TGGTGA(T/G)AG-3′. A homologous sequence to each of the three common sequences is also found in the 5′-flanking regions of the genes for the β subunit of human F0F1-ATPase and rat somatic cytochrome c. It may be that the common sequences play an important role in the coordinate expression of nuclear genes encoding mitochondrial proteins responsible for oxidative phosphorylation. Southern blot analyses showed that both genes are present in a single copy in the human genome and are located on chromosome 8.
KeywordsFlank Region Nuclear Gene Sense Strand Antisense Strand Common Sequence
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