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Bioenergetics pp 327-340 | Cite as

Gene Structure of Human ATP Synthase Beta Subunit

  • Yasuo Kagawa
  • Shigeo Ohta

Summary

The β subunit of ATP synthase is a catalytic subunit that is highly homologous among different species. Human nuclear genes for the mitochondrial enzymes including the β subunits and 8 related proteins were cloned to elucidate the coordinated transcriptional control of energy transduction.

The human β gene (8kbp) contained 10 exons and 8 Alu repeating sequences, without any TATA box. An S1 nuclease protection experiment revealed two initiation sites for the transcription. The promoter activity of the upstream region was examined by transfecting HeLa cells with a chloramphenicol acetyltransferase (CAT) gene ligated with various lengths of the 5′ upstream region of the β gene. The regions with promoter activity were analyzed by DNase I foot printing and the gel retardation assay. We found a special enhancer with the motif GAGACAAGGTTTCACC and the transcriptional factor(s) to coordinate the expression of some mitochondrial energy transducing enzymes.

Keywords

Pyruvate Dehydrogenase Energy Transduce Chloramphenicol Acetyl Transferase Chloramphenicol Acetyl Transferase Activity Chloramphenicol Acetyl Transferase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Yasuo Kagawa
    • 1
  • Shigeo Ohta
    • 1
  1. 1.Department of BiochemistryJichi Medical SchoolMinamikawachi, Tochigi-kenJapan

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