Bioenergetics pp 285-293 | Cite as

High Pressure Kinetic Studies on Certain Hemoproteins

  • Claude Balny


Pressure, as a perturbing agent, is one of the most powerful tools to investigate the thermodynamic parameters of biochemical (and chemical) reactions. By using the transition state theory, the thermodynamic quantity ΔV‡ was determined under different experimental conditions for some reactions involving various heme proteins. Results are discussed in terms of changes in protein conformation induced by changes in solvent, temperature or pressure which affected the reactions rates. These were determined using the high-pressure low-temperatures stopped-flow method.


Activation Volume Slow Phase Heme Protein Transition State Theory Ammonia Monooxygenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

The abbreviations used are


horseradish peroxidase


hydroxylamine oxidoreductase


cytochrome c peroxidase


ethyl peroxide


ethylene glycol


dimethyl sulfoxide


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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Claude Balny
    • 1
  1. 1.Institut National de la Santé et de la Recherche MédicaleINSERM U 128 - CNRSMontpellier CedexFrance

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