Theory and Applications of the Kinetics of Substrate Reaction during Irreversible Modification of Enzyme Activity
Kinetics of enzyme inhibition has been studied systematically and from a unified schemeit has been shown that the concept of substrate competition applied toboth reversible and irrreversible inhibitions. The effect of substrate on the inhibition rate constants can be used as kinetic criteria for irreversible inhibitions. Equations are presented for the substrate reaction in the presence of the modifier and it is shown that the apparent rate constant, A, for irreversible modification reactions can be determined in one experiment by following the formation of products in presence of the inhibitors. Experimental examples are given to show that constants thus obtained are generally in agreement with those given by conventional methods. The above approach for modification kinetics has been applied to enzyme inactivation during denaturation, enzyme activation, slow reversible inhibition and enzyme reactions involving two substrates.
KeywordsCreatine Kinase Substrate Reaction Free Enzyme Apparent Rate Constant Irreversible Inhibition
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