Molecular Organization and Regulation of the Protonmotive System of Mammalian ATP Synthase
In bacteria the H+ translocating membrane sector (FO) of the F0, F1 H+ ATP synthase is composed of three subunits (a, b, and c of the E. Coli enzyme (1). In mitochondria more than three proteins appear to constitute the Fo and stalk sectors (1), which are involved in H+ conduction and/or coupling of H+ translocation to the catalytic process in the F1 moiety (Table 1) (2,3). Two of these are the products of the mitochondrial genoma:the ATPase 6, homologous to subunit a of E. Coli and likely to be involved as this (4–7) in H+ conduction, and A6L in mammals (aapl in yeast), whose function is as yet unknown (1). Other 5 proteins have been identified in the mammalian enzyme which are encoded by nuclear genes (8,9). These include subunit c which is directly involved in H+ conduction (10,11), OSCP and F6 which contribute to connection of F1 with FO (1).
KeywordsATPase Activity Proton Conduction Trypsin Digestion Particle Protein Submitochondrial Particle
Unable to display preview. Download preview PDF.
- 2.Papa, S. (1989) in “Organelles of Eukaryotic Cells:Molecular Structure and Interactions” (Tager, J.M., Guerrieri, F., Azzi, A. and Papa, S. eds.) Plenum Press, N.Y. (in press).Google Scholar
- 3.Papa, S., Guerrieri, F., Zanotti, F., Houstek, J., Capozza, G. and Ronchi, S., (1988) in “Molecular Basis of Biomembrane Transport” (Palmieri, F. and Quagliariello, E.,eds.) Elsevier Science Publisher BV, Amsterdam, pp.249–259.Google Scholar
- 4.Von Meyenburg, K., Jørgensen, B.B., Michelsen, O., Sørensen, L. and McCarty, J.E.C. (1986) EMBO Journal 4, 2357–2362.Google Scholar
- 6.Eya, S., Noumit, P., Maede, M. and Futai, M. (1988) The Journal of Biol. Chem.263, 10056–10062.Google Scholar
- 27.Sanadi, D.R. (1982) Biochim.Biophys.Acta 683, 39–56Google Scholar
- 29.Perlin, D.S., Cox, D.N. and Senior, A.E. (1983), J. Biol. Chem.258, 9713–9800.Google Scholar
- 30.Ario, J.P., Klionsky, J. and Simoni, R.D. (1985) J. Biol. Chem.260, 11207–11215.Google Scholar