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Astrocytes as Antigen Presenting Cells for Primary and Secondary T Cell Responses: Effect of Astrocyte Infection by Murine Hepatitis Virus

  • Rainald Mößner
  • Jonathon Sedgwick
  • Egbert Flory
  • Heiner Körner
  • Helmut Wege
  • Volker ter Meulen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)

Abstract

CD4+ T cell lines specific for murine hepatitis virus (MHV)-JHM or myelin basic protein (MBP) proliferated when cultured together with MHC class I and II positive syngeneic rat astrocytes and either inactivated virus or MBP as antigen. The magnitude of the T cell proliferative response was comparable to that seen when thymocytes were used as a source of antigen presenting cells (APC).

In contrast, MHC class I and II positive astrocytes were unable to significantly stimulate the proliferation of highly purified populations of naive CD4+ and CD8+ T cells in an allogeneic mixed lymphocyte reaction (MLR). Both T cell populations proliferated when mixed with allogeneic lymph node cells. Infection of the astrocytes with a variant of MHV-JHM (PI-AS22D) did not alter this cells incapacity to stimulate the naive CD4+ and CD8+ T cells to proliferate.

Keywords

Myelin Basic Protein Accessory Cell Mixed Lymphocyte Reaction Brown Norway Bone Marrow Origin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Rainald Mößner
    • 1
  • Jonathon Sedgwick
    • 1
  • Egbert Flory
    • 1
  • Heiner Körner
    • 1
  • Helmut Wege
    • 1
  • Volker ter Meulen
    • 1
  1. 1.Institute for Virology and ImmunobiologyUniversity of WürzburgWürzburgWest Germany

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