Quantitation, Phenotypic Characterization and In Situ Localization of Lymphoid Cells in the Brain Parenchyma of Rats with Differing Susceptibility to Coronavirus JHM-Induced Encephalomyelitis
Intracerebral infection of rats with Coronavirus JHM may cause multiple neurological syndromes ranging from acute lethal encephalitis to subclinical demyelination1,2. The outcome of the infection is determined by both the type of virus used for inoculation as well as the genetic background of the host animal1,2,3,4. Data accumulated from different inbred strains of rats suggest that important host factors which influence the course of the disease include the maturation of viral target cells5 and the state of immunocompetence of the animal6,7. Although virus-specific immunity in the cerebrospinal fluid of affected animals has been examined repeatedly in the past8,9,10, little information has been collected about the dynamics of immune reactions taking place in the brain parenchyma. However, these local immunological events are almost certainly decisive for the clinical course of the infection. Therefore, we attempted to analyse the kinetics of the lymphoid cell infiltration following intracerebral inoculation of two rat strains with coronvirus JHM. In order to detect relationships between neurological symptomatology and local immune reactions in the brain tissue we selected two inbred strains which are known to behave differently after intracerebral infection. Whereas Lewis rats often develop a subacute demyelinating encephalomyelitis accompanied by severe paralytic signs of disease, Brown Norway (BN) rats reveal no signs whatsoever, although small foci of nodular demyelination can be detected in periventricular areas of the brain2.
KeywordsBrown Norway Intracerebral Inoculation Neurological Symptomatology Paralytic Disease Lymphoid Cell Infiltration
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