Vacuolar Encephalomyelopathy in Mice Induced by Intracerebral Inoculation with a Coronavirus JHM-CC Strain
JHM-CC virus, a mutant strain of mouse hepatitis virus, was isolated by Hirano in 1980 from a DBT cell culture persistently infected with the neurovirulent strain, JHM1. This mutant strain is characterized by formation of smaller plaques on the DBT cells and by lower cell virulence than the original JHM1. Although several murine coronaviruses such as JHM are known to cause demyelinating encephalitis2,4, we found that JHM-CC does not induce primary demyelination, but instead produces characteristic vacuolar degeneration in the brain and spinal cord. In this report we describe the clinical manifestations, neuropathological changes and viral antigen distribution in the central nervous system (CNS) of ICR mice infected intracerebrally with JHM-CC.
KeywordsViral Antigen Vacuolar Degeneration Mouse Hepatitis Virus Habenular Nucleus Vacuolar Change
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