MHV-Resistant SJL/J Mice Express a Non-Functional Homolog to the MHV Receptor Glycoprotein
MHV-A59 recognizes a 100–110 K glycoprotein receptor on intestinal and liver plasma membranes. The receptor protein was affinity purified using an antireceptor monoclonal antibody. Purified receptor was sequenced from the 1st to the 15th amino acids. Antiserum raised against a synthetic peptide having this sequence bound to the 110 K receptor from BALB/c intestine and a 58 K fragment of the receptor. SJL/J mice are resistant to MHV-A59 and their plasma membranes were previously shown to lack binding to MHV. However, antipeptide antibody bound to a 105 K protein and a 53 K protein fragment from SJL/J plasma membranes. These SJL/J proteins did not bind MHV or to the blocking monoclonal anti-receptor antibody. Genetic resistance of SJL/J mice to MHV-A59 appears to be due to mutation of the receptor glycoprotein resulting in a molecule that does not bind MHV.
KeywordsVariant Protein Liver Plasma Membrane Complete Amino Acid Sequence Virus Binding Mouse Hepatitis Virus
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