Characterization and Location of the Structural Polypeptides of Turkey Enteric Coronavirus Using Monoclonal Antibodies and Enzymatic Treatments
Turkey enteric Coronavirus (TCV) is one of the major causes of epidemic diarrhoea in turkey poults (1, 2). The morphological and physicochemical characteristics of TCV resemble those of other members of the family Coronaviridae (3, 4). However, little is known with respect to the molecular and antigenic structure of the TCV virion, due to difficulties in propagating TCV strains in tissue cultures and lack of highly specific immunological probes (1, 4). Field isolates can be propagated by oral inoculation and intestinal infections of young turkey poults, or by inoculation into embryonating turkey eggs (4, 5). TCV possesses a hemagglutinating (HA) activity which may be associated to short granular projections located near the base of the characteristic larger bulbous peplomers (6). Recently, we adapted TCV isolates in HRT-18 cells, an established cell line derived from human rectum adenocarcinoma (7). In these cells, TCV induces cytopathic changes, including polykaryocytosis, which depended on trypsin in the culture medium (8).
KeywordsInfectious Bronchitis Virus Western Immunoblotting Infectious Bronchitis Virus Strain Polypeptide Structure Turkey Poult
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- 1.B. S. Pomeroy. Coronaviral enteritis of turkeys, in: Disease of poultry, 8th ed. M. S. Holstad, H. J. Barnes, B. W. Calnek, W. M. Reid, and H. W. Yoder, ed., Iowa State Univ. Press, Ames (1984).Google Scholar
- 3.D. R. Deshmukh and B. S. Pomeroy. Physicochemical characterization of a bluecomb Coronavirus of turkeys. Am. J. Vet. Res. 35: 1549 (1977).Google Scholar
- 7.W. A. F. Tompkins, A. W. Watrach, J.D. Schmale, R.M. Schultz, and J.A. Harris. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52:101 (1974).Google Scholar
- 8.S. Dea, S. Garzon, and P. Tijssen. Isolation and trypsin-enhanced propagation of turkey enteric (Bluecomb) coronaviruses in a continuous human rectal tumor (HRT-18) cell line. Am. J. Vet. Res. (In press).Google Scholar
- 9.S. Dea and P. Tijssen. Antigenic and polypeptide structure of turkeyenteric coronaviruses as defined by monoclonal antibodies. J. Gen. Virol. (In press).Google Scholar
- 11.H. Laude, J.M. Chapsal, J. Gelfi, S. Labiau, and J. GrosClaude. Antigenic structure of transmissible gastroenteritis virus. I. Properties of monoclonal antibodies directed against virion proteins. J. Gen. Virol. 67:119 (1986).Google Scholar
- 13.S. Dea, S. Garzon, and P. Tijssen. Intracellular synthesis and processing of the structural glycoproteins of turkey enteric Coronavirus. Arch. Virol. (In press).Google Scholar
- 15.D. Cavanagh, P. J. Davis, D. J. C. Pappin, M. M. Binns, M. E. G. Boursnell, and T. D. K. Brown. Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor polypeptide of IBV strains Beaudette and M41. Virus Res. 4:133 (1986).PubMedCrossRefGoogle Scholar
- 20.L. S. Sturman and K. V. Holmes. The molecular biology of coronaviruses. Adv. Virus Res. 56:904 (1983).Google Scholar