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Structure and Function Studies of the Nucleocapsid Protein of Mouse Hepatitis Virus

  • Paul S. Masters
  • Monica M. Parker
  • Cynthia S. Ricard
  • Cynthia Duchala
  • Mark F. Frana
  • Kathryn V. Holmes
  • Lawrence S. Sturman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)

Abstract

Coronaviruses, like virtually all single-stranded RNA viruses, contain a nucleocapsid (N) protein in close structural association with their genomes.1 Knowledge of the structure and functions of N proteins would be fundamental to an understanding of the viral life cycle and pathogenesis. We have undertaken a study of the N protein of mouse hepatitis virus (MHV) in an attempt to learn the roles of this protein in viral assembly, transcription and translation. Specifically, we would like to examine: (i) the nature of the interaction between N protein and the genome RNA; (ii) the nature of the interactions between adjacent and more distant pairs of N monomers in the helically symmetric viral nucleocapsid; and (iii) the location and purpose of the phosphoserine residue(s) known to occur in the N molecule.2,3 To date, we have pursued three approaches to these questions: (i) a comparison of the sequences of the N proteins of different, independently isolated strains of MHV; (ii) the examination of an N protein mutant of MHV-A59, Albany-4; and (iii) the in vitro expression and functional assay of N protein and of engineered mutants of N protein. Our results suggest that the MHV N protein has three structural domains, at least two of which may be functionally distinct.

Keywords

Infectious Bronchitis Virus Nucleocapsid Protein Viral Life Cycle Nonpermissive Temperature Bromphenol Blue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Paul S. Masters
    • 1
  • Monica M. Parker
    • 1
  • Cynthia S. Ricard
    • 1
  • Cynthia Duchala
    • 2
  • Mark F. Frana
    • 2
  • Kathryn V. Holmes
    • 2
  • Lawrence S. Sturman
    • 1
  1. 1.Wadsworth Center for Laboratories and ResearchNew York State Department of HealthAlbanyUSA
  2. 2.Uniformed Services University of the Health SciencesBethesdaUSA

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