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Differential Reactivity of Bovine Coronavirus (BCV) and Influenza C Virus with N-Acetyl-9-O-Acetylneuraminic Acid (NEU5,9AC2)-Containing Receptors

  • Beate Schultze
  • Hans-Jürgen Groβ
  • Hans-Dieter Klenk
  • Reinhard Brossmer
  • Georg Herrler
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)

Abstract

Only little information is available about the initial events in the infectious cycle of coronaviruses. Attachment of strain A59 of mouse hepatitis virus to the cell surface has been shown to be mediated by a protein of about 110 kDalton (Boyle et al., 1988). Recently, bovine Coronavirus (BCV) and human corona-virus OC43 (HCV-OC43) have been shown to recognize receptors on erythrocytes similar to influenza C virus. The evidence is based on the finding that both influenza C virus and BCV contain a sialate O-acetylesterase, which functions as a receptor-destroying enzyme (Vlasak et al, 1988). Incubation of chicken red blood cells with the esterase of either virus renders the erythrocytes resistant to agglutination by both viruses as well as by HCV-OC43. This result indicates that N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) is a receptor determinant for attachment of BCV and HCV-OC43 to erythrocytes as has been shown previously for influenza C virus (Rogers et al., 1986). Here we confirm the importance of Neu5,9Ac2 for the binding of BCV to the cell surface by resialylation of erythrocytes. We present evidence that strain Johannesburg/1/66 of influenza C virus is more efficient in recognizing Neu5,9Ac2-containing receptors than BCV.

Keywords

Sialic Acid Human Erythrocyte Mouse Hepatitis Virus Hemagglutination Titer Bovine Coronavirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Beate Schultze
    • 1
  • Hans-Jürgen Groβ
    • 2
  • Hans-Dieter Klenk
    • 1
  • Reinhard Brossmer
    • 2
  • Georg Herrler
    • 1
  1. 1.Institut für VirologiePhilipps-Universität MarburgDeutschland
  2. 2.Institut für Biochemie IIUniversität HeidelbergDeutschland

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