On the Membrane Cytopathology of Mouse Hepatitis Virus Infection as Probed by a Semi-Permeable Translation-Inhibiting Drug

  • G. Macintyre
  • C. Kooi
  • F. Wong
  • R. Anderson
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)


Previous studies of the membrane fusion process have permitted the characterization of membrane permeability changes concomitant with MHV-induced cytopathology. One indication of membrane permeability in MHV-infected cells is their sensitivity to translational inhibition by the normally impermeable amino-glycoside, hygromycin B (Macintyre, G., Wong, F. and Anderson, R. (1989) J. Gen. Virol. 70, 763–768). In the present study, we examine the hygromycin B sensitivity of acutely infected mouse fibroblast L-2 cell and macrophage cultures as well as persistently infected mouse fibroblast LM-K cell cultures. The results suggest that membrane permeability alterations (as indicated by hygromycin B sensitivity) are a common feature of these MHV infections. Hygromycin B “cured” persistently infected LM-K cells as indicated by the absence of detectable virus antigen by immunofluorescence and by the absence of infectious virus even after removal of the drug or co-cultivation with untreated L-2 cells. The results argue against the maintenance of MHV infection by a mechanism involving latently or non-cytolytically infected cells. We conclude therefore that at least one mechanism for MHV persistence depends on virus propagation by cytolytic infection of a small, dynamically changing, fraction of the total cells present in culture.


Minimal Essential Medium Peritoneal Macrophage Viral Antigen Infectious Virus Demyelinating Disease 


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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • G. Macintyre
    • 1
  • C. Kooi
    • 1
  • F. Wong
    • 1
  • R. Anderson
    • 1
  1. 1.Department of Microbiology & Infectious DiseasesUniversity of CalgaryCalgaryCanada

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