Pharmacological Control of Phospholipase A2 Activity In Vitro and In Vivo
Phospholipase(s)A2 (PLA2) are a class of enzymes that catalyze the hydrolysis of membrane phospholipids to liberate free fatty acids from the sn-2 position resulting in the formation of free fatty acids, predominantly arachidonic acid, and lysophospholipid. Within the last 20 years their role in disease has gained increasing attention (1,2,3). Various physiological stimuli (antigen-antibody complexes, cytokines, angiotensin II, bradykinin, prolactin and thrombin) activate PLA2 when added to responsive cells. Indeed, high levels of PLA2 activity have been found in joint fluid of patients with rheumatoid arthritis, in serum of patients with endotoxin shock or pancreatitis, in psoriatic lesions or peritoneal lavage fluids of patients with bacterial peritonitis. In some cases, enhanced levels of PLA2 correlate with disease severity and have been proposed for use as a biochemical marker of disease activity (4,5).
KeywordsPlatelet Activate Factor Snake Venom Aristolochic Acid PLA2 Activity Peritoneal Lavage Fluid
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