The Uppsala Method for Rapid Analysis of Total Dietary Fiber
An understanding of the nutritional effects of dietary fiber has been considerably hampered by the lack of an appropriate definition, and consequently of adequate analysis methods, for this component. Based on physiological criteria, Trowell (1972) defined dietary fiber as “the remnants of the plant cell-wall that are not hydrolysed by the alimentary enzymes of man”, and this was later simplified and expanded to include “the plant polysaccharides and lignin which are resistant to hydrolysis by the enzymes of man” (Trowell et al., 1976). In 1979 we proposed that dietary fiber could be defined as the sum of non-starchy polysaccharides and Klason lignin, and, in conjunction with this chemical definition, published the first method (the Uppsala method) for the analysis and characterization of fibers (Theander and Åman, 1979). As this analytical procedure includes ‘starch’ resistant to α-amylases and, as part of the Klason lignin complex, other indigestible components such as tannins, cutins and Maillard products, this definition conforms well with the original definitions of Trowell and co-workers.
KeywordsResistant Starch Soluble Fiber Total Dietary Fiber Klason Lignin Soluble Dietary Fiber
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