Issues in Dietary Fiber Analysis

  • Judith A. Marlett
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 270)


There are many reasons for measuring dietary fiber. We have three overall aims in our laboratory. First, analysis of the major sources of dietary fiber in the USA food supply would provide a data base of values that could be used clinically and epidemiologically to assess the kinds and amounts of dietary fiber consumed by individuals and populations. Second, the content and composition of dietary fiber sources can be related to their physiological effects. For example, evidence has accumulated rapidly in recent years to suggest many soluble dietary fiber sources have physiological effects primarily in the upper part of the gastrointestinal tract, whereas insoluble sources influence the physiology of the lower gut (1). Ultimately, researchers want to understand the mechanisms by which dietary fiber has a particular physiological effect; the composition of dietary fiber sources, as well as their physiochemical properties, will provide insight into understanding these mechanisms of action for dietary fiber.


Neutral Sugar Kidney Bean Total Dietary Fiber Total Fiber Soluble Dietary Fiber 
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  1. 1.
    Pilch S. (ed.) Physiological Effects and Health Consequences of Dietary Fiber. FASEB, Bethesda, Maryland, 1987.Google Scholar
  2. 2.
    Marlett JA. Analysis of Dietary Fiber in Human Foods, in: Third Vahouny Conference on Dietary Fiber in Health and Disease, (D. Kritchevsky, ed.) April, 1988, Washington, D.C. (In press)Google Scholar
  3. 3.
    Van Soest PJ, Wine RH. Use of detergents in the analysis of fibrous foods. IV. Determination of plant cell-wall constituents. J. Assoc. off. Anal. Chem. 50:50–55, 1967.Google Scholar
  4. 4.
    Robertson JB, Van Soest PJ. The detergent system of analysis and its application to human foods, in: The Analysis of Dietary Fiber in Food James WP1, Theander O. (eds.) Marcel Dekker, Inc., New York, pp 123–158, 1981.Google Scholar
  5. 5.
    Prosky L, Asp NG, Furda I, et al. Determination of total dietary fiber in foods and food products: collaborative study. J. Assoc. Off. Anal. Chem. 68:677–679, 1985.Google Scholar
  6. 6.
    Prosky L, Asp, NG, Schweizer TF, et al. Determination of insoluble, soluble and total dietary fiber in foods and food products: interlaboratory study. J. Assoc. Off. Anal. Chem. 71:1017–1023, 1988.Google Scholar
  7. 7.
    Asp NG, Johansson CG, Hallmer H, Siljestrom M. Rapid enzymatic assay of insoluble and soluble dietary fiber. J. Agric. Food Chem. 31:476–482, 1983.CrossRefGoogle Scholar
  8. 8.
    Mongeau R, Brassard R. A rapid method for the determination of soluble and insoluble dietary fiber: comparison with AOAC total dietary fiber procedure and Englyst’s method. J. Food Sci. 51:1333–1336, 1986.CrossRefGoogle Scholar
  9. 9.
    Aman P, Graham H. Analysis of total and insoluble mixed linked (1,3), (1,4)-ß-D-glucans in barley and oats. J. Agric. Food Chem. 35:704–709, 1987.CrossRefGoogle Scholar
  10. 10.
    Shinnick FL, Longacre MJ, Ink SL, Marlett JA. Oat fiber: composition vs. physiological function. J. Nutr. 118:144–151, 1988.Google Scholar
  11. 11.
    Theander O, Aman P. Studies on dietary fibers. 1. Analysis and chemical characterization of water-soluble and water-insoluble dietary fibers. Swed. J. Agric. Res. 9:97–106, 1979.Google Scholar
  12. 12.
    Theander O, Westerlund E. Studies on dietary fiber. 3. Improved procedures for analysis of dietary fiber. J. Agric. Food Chem. 34:330–336, 1986.CrossRefGoogle Scholar
  13. 13.
    Neilson MJ, Marlett JA. A comparison between detergent and nondetergent analyses of dietary fiber in human foodstuffs, using high-performance liquid chromatography to measure neutral sugar composition. J. Agric. Food Chem. 31:1342–1347, 1983.CrossRefGoogle Scholar
  14. 14.
    Marlett, J.A., Chester, J.G., Longacre, M.J., and Bogdanske, J.J. Recovery of Soluble Dietary Fiber is Dependent on the Method of Analysis. Am. J. Clin. Nutr. 50:479–485, 1989.Google Scholar
  15. 15.
    Englyst HN, Wiggins HS, Cummings JH. Determination of non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 107:307–318, 1982.CrossRefGoogle Scholar
  16. 16.
    Englyst HN, Hudson GJ. Colorimetric method for routine measurement of dietary fibre as non-starch polysaccharides. A comparison with gas-liquid chromatography. Food Chem. 24:63–76, 1987.CrossRefGoogle Scholar
  17. 17.
    Cummings JH, Englyst HN. The development of methods for the measurement of dietary fibre in food, in: Morton ID. (ed.) Cereals in a European Context: First European Conference on Food Science and Technology. New York: VCH Publishers, Inc., pp 188–220, 1987.Google Scholar
  18. 18.
    Southgate DAl. Determination of carbohydrates in foods. II. Unavailable carbohydrates. J. Sci. Fd. Agric. 20:332–335, 1969.Google Scholar
  19. 19.
    Southgate DAl. Use of the Southgate method for unavailable carbohydrates in the measurement of dietary fiber, in: The Analysis of Dietary Fiber in Food, James WPl, Theander O. (ed.) Marcel Dekker, Inc., New York, pp 1–19, 1981.Google Scholar
  20. 20.
    Marlett JA, Navis D. Comparison of gravimetric and chemical analyses of total dietary fiber in human food. J. Agric. Food Chem. 36:311–315, 1988.CrossRefGoogle Scholar
  21. 21.
    Larm D, Theander O, Aman P. Structural studies on a water-soluble arabinan isolated from rapeseed (Brassica Napus), Acta Chem. Scand., B29:1011–1014, 1975.CrossRefGoogle Scholar
  22. 22.
    Marlett JA. Analysis of dietary fiber. Animal. Feed Sci. and Tech. 23:1–13, 1989.CrossRefGoogle Scholar
  23. 23.
    Shinnick FL, Hess RL, Fischer MH, Marlett JA. Apparent Nutrient Absorption and Upper Gastrointestinal Transit with Fiber-Containing Enterai Feedings Am. J. Clin. Nutr. 49:471–475, 1989.Google Scholar
  24. 24.
    Anderson JW, Bridges SP. Dietary fiber content of selected food. Am. J. Clin. Nutr. 47:440–447, 1988.Google Scholar
  25. 25.
    Theander O, Aman P. Analysis of dietary fibers and their main constituents, in: The Analysis of Dietary Fiber in Food, James WPl, Theander O. (eds.), Marcel Dekker, Inc., New York, pp 51–70, 1981.Google Scholar
  26. 26.
    Stephen AM, Cummings JH. Water-holding by dietary fibre in vitro and its relationship to faecal output in man. Gut 20:722–729, 1979.CrossRefGoogle Scholar
  27. 27.
    Slavin JL, Marlett JA. Influence of refined cellulose on human bowel function and calcium and magnesium balance. Am. J. Clin. Nutr. 33:1932–1939, 1980.Google Scholar
  28. 28.
    Cummings JH, Southgate DAl, Branch WJ et al. The digestion of pectin in the human gut and its effect on calcium absorption and large bowel function. Br. J. Nutr. 41:477–485, 1979.CrossRefGoogle Scholar
  29. 29.
    Marlett JA. Dietary fiber and mineral bioavailability. IM-Int. Med. Specialist 5:99–114, 1984.Google Scholar
  30. 30.
    Johansson C, Aman P, Asp N et al. Enzymatic and neutral detergent fibre methods for dietary fibre analysis. Swed. J. Aqric. Res. 12:157–161, 1982.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Judith A. Marlett
    • 1
  1. 1.Department of Nutritional SciencesUniversity of Wisconsin-MadisonMadisonUSA

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