Polysaccharide Utilization by Human Colonic Bacteria

  • Abigail A. Salyers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 270)

Abstract

A complex and constantly changing mixture of polysaccharides enters the human colon every day. Most of these polysaccharides are plant cell wall polysaccharides. Although plant cell wall polysaccharides can be degraded to a limited extent by exposure to acid in the stomach, they are not digested at all by human small intestinal enzymes and thus reach the colon virtually intact. Also included in the mixture that enters the colon are host polysaccharides such as glycoprotein raucins secreted by goblet cells and mucopolysaccharides released during sloughing of intestinal mucosal cells. Since the rate of mucin production and mucosal cell turnover increases as the amount of fiber in the diet increases, the amount of host polysaccharide entering the colon is not constant but varies with the composition of the diet (Vahouny and Cassidy, 1986).

Keywords

Cellulose Fermentation Starch Lignin Polysaccharide 

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References

  1. Anderson, K. and Salyers, A. A., 1989a, Biochemical evidence that starch breakdown by Bacteroides thetaiotaomicron involves outer membrane starch binding sites and periplasmic starch degrading enzymes, J. Bacteriol., 171: 3192.Google Scholar
  2. Anderson, K., and Salyers, A, A., 1989b, Genetic evidence that outer membrane binding of starch is required for starch utilization by Bacteroides thetaiotaomicron, J.’ Bacteriol., 171: 3199.Google Scholar
  3. Ehle, F. R., Robertson, J. B., and VanSoest, P. J., 1982, Influence of dietary fiber on fermentation in the human large intestine, J. Nutr., 112: 158.Google Scholar
  4. Englyst, H. N., and Cummings, J. S., 1985, Digestion of the polysaccharides in some cereal foods in the human small intestine, Am. J. Clin. Nutr., 42: 778.Google Scholar
  5. Gibson, G. R., Cummings, J. H., and Macfarlane, G. T., 1988a, Competition for hydrogen between sulphate-reducing bacteria and methanogenic bacteria from the human large intestine, J. Appl. Bacteriol., 65:241.CrossRefGoogle Scholar
  6. Gibson, G. R., Macfarlane, G. T., and Cummings, J. H., 1988b, Occurrence of sulphate-reducing bacteria in human feces and the relationship of dissimilatory sulphate reduction to methanogenesis in the large gut, J. Appl. Bacteriol., 65: 103.CrossRefGoogle Scholar
  7. Gibson, G. R., Cummings, J. H., and Macfarlane, G. T., 1988c, Use of a three-stage continuous culture system to study the effect of mucin on dissimilatory sulfate reduction and methanogenesis by mixed populations of human gut bacteria, Appl. Environ. Microbiol., 54: 2750.Google Scholar
  8. Hylemon, P. B., and Glass, T. L., 1983, Biotransformation of bile acids and cholesterol by the intestinal microflora, in: Human Intestinal Microflora in Health and Disease, D. Hentges, ed., Academic Press, New York, pp. 189–214.Google Scholar
  9. McCarthy, R. E., and Salyers, A. A., 1988, Effect of dietary fiber utilization on the colonic microflora, in: “The Role of the Gut Flora in Toxicity and Cancer,” I. Rowland, ed., Academic Press, London, pp. 295–313.Google Scholar
  10. Moore, W. E. C., Cato, E. P., and Holdeman, L. V., 1977, Some current concepts in intestinal bacteriology, Amer. J. Clin. Nutr., 31: S33.Google Scholar
  11. Salyers, A. A., and Pajeau, M., Competitiveness of different polysaccharide utilization mutants of Bacteroides thetaiotaomicron in the intestinal tracts of germfree mice, Appl. Environ. Microbiol., in press.Google Scholar
  12. Salyers, A. A., Pajeau, M. P., McCarthy, R. M., 1988, Importance of mucopolysaccharides as substrates for Bacteroides thetaiotaomicron growing in the intestinal tracts of germfree mice, Appl. Environ. Microbiol., 54: 1970.Google Scholar
  13. Salyers, A. A., Vercellotti, J. R., West, S. E., and Wilkins, T. D., 1977, Fermentation of mucin and plant polysaccharides by strains of Bacteroides from the human colon, Appl. Environ. Microbiol., 33: 319.Google Scholar
  14. Salyers, A. A., West, S. E., Vercellotti, J. R., and Wilkins, T. D., 1978, Fermentation of mucin and plant polysaccharides by anaerobic bacteria from the human colon, Appl. Environ. Microbiol., 34: 529.Google Scholar
  15. Smith, K., and Salyers, A. A., 1989, Characterization of a cellassociated Bacteroides pullulanase and determination of its contribution to pullulan utilization, J. Bacteriol., 171: 2116.Google Scholar
  16. Stahl, D. A., 1988, Phylogenetically based studies of microbial ecosystem perturbation, in_ “Biotechnology for Crop Protection,” P. A. Hedin, J. L. Menn and R. M. Hollingworth, eds., American Chemical Society Press, pp. 373–390.Google Scholar
  17. Vahouny, G. V., and Cassidy, M. M., 1986, Dietary fiber and intestinal adaptation, in: “Dietary Fiber: Basic and Clinical Aspects,” G. V. Vahouny, D. Kritchevsky, eds., Plenum, New York, pp. 181–210.Google Scholar
  18. Van Soest, P. J., 1978, Dietary fibers: their definition and nutritional properties, Amer. J. Clin. Nutr., 31: 512.Google Scholar
  19. Vercellotti, J. R., Salyers, A. A., Bullard, W. S., and Wilkins, T. D., 1977, Breakdown of mucin and plant polysaccharides in the human colon. Canad. J. Biochem., 55: 1190.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Abigail A. Salyers
    • 1
  1. 1.Department of MicrobiologyUniversity of IllinoisUrbanaUSA

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