Generation of Non MHC Restricted Cytotoxic Immune Responses: Effects of “in Vitro” Hyperthermic Treatment
Hyperthermia represents a promising approach to the therapy of neoplastic diseases. Application of local hyperthermia to a tumor has been suggested as a method to selectively enhance the action of chemotherapeutic agents at the tumor site (1). Moreover evidence suggests that hyperthermia by itself or in conjunction with X rays produce an increase in the cure rates of human tumors (2). Many studies support the hypothesis that the plasma membrane is a critical target for heat cell inactivation. Hyperthermia has been reported to induce structural changes in cell membranes (3–5). The mechanisms involved in hyperthermic action have been reviewed extensively (6,7) but little is known about the effects of hyperthermic treatment on immune response. As many recent data enlight the effectiveness of adoptive immunotherapy protocols in the control of neoplastic diseases (8,9), we sought to investigate the feasibility of a combined-therapeutic approach including hyperthermia and adoptive immunotherapy. In this line we studied the effects of hyperthermic treatment on basic immune responses, such as anti T3-Ti triggering induced lymphoproliferation, and the generation of non MHC-restricted cytotoxic activity against tumor cells.
KeywordsPeripheral Blood Mononuclear Cell Adoptive Immunotherapy Hyperthermic Treatment K562 Target Cell Peripheral Blood Mononuclear Cell Preparation
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