Plant Aging pp 75-80 | Cite as

Factors Affecting Tissue Culture Success in Mass Propagation

  • Pier-Luigi Pasqualetto
Part of the NATO ASI Series book series (NSSA, volume 186)


Tissue culture techniques are a wonderful and powerful tool in the hands of plant propagators. Micropropagation is probably the most widely applied tissue culture technique and its direct impact on commercial plant production is considerable (Debergh, 1987). Since Morel and Martin obtained virus-free dahlias by meristem culture in 1952, tissue culture techniques have progressed significantly. The list of plants vegetatively propagated in vitro has increased in recent years, though many difficulties and problems still remain to be solved. In this paper I deal with the major factors affecting tissue culture success in a mass propagation system, discussing its in vitro and in vivo aspects.


Somatic Embryogenesis Woody Plant Adventitious Shoot Mother Plant Apical Dominance 


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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Pier-Luigi Pasqualetto
    • 1
  1. 1.Azienda Agricola Meristema SRLLaboratorio di micropropagazioneCascine di Butl (Pisa)Italy

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