Growth and Development in in Vitro Long Term Cultures
Long term storage and preservation of experimental material is useful for in vitro research. However, callus cultures of most plant species lose their regeneration capacity with age in culture (Gould, 1986; Halperin, 1986). In tobacco callus, that capacity is lost slowly, beginning during the second year in culture (Murashige and Nakano, 1967) and being manifested by a slower formation of shoots, followed by a sequential reduction in the number of shoots formed per callus and finally by little or no shoot formation (Thorpe, unpublished). It is desirable to use the same tissue line from year to year when investigating physiological parameters of morphogenesis. This would eliminate introduction of different genotypes and allow comparisons of previous data without fear that differences are arising from different callus lines.
KeywordsSomatic Embryo Somatic Embryogenesis Callus Culture Shoot Formation Callus Line
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