Plant Aging pp 399-403 | Cite as

Growth and Development in in Vitro Long Term Cultures

  • Richard W. JoyIV
  • Trevor A. Thorpe
Part of the NATO ASI Series book series (NSSA, volume 186)


Long term storage and preservation of experimental material is useful for in vitro research. However, callus cultures of most plant species lose their regeneration capacity with age in culture (Gould, 1986; Halperin, 1986). In tobacco callus, that capacity is lost slowly, beginning during the second year in culture (Murashige and Nakano, 1967) and being manifested by a slower formation of shoots, followed by a sequential reduction in the number of shoots formed per callus and finally by little or no shoot formation (Thorpe, unpublished). It is desirable to use the same tissue line from year to year when investigating physiological parameters of morphogenesis. This would eliminate introduction of different genotypes and allow comparisons of previous data without fear that differences are arising from different callus lines.


Somatic Embryo Somatic Embryogenesis Callus Culture Shoot Formation Callus Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Gould, A. F., 1986, Factors controlling generation of viability in vitro, in: “Cell Culture and Somatic Cell Genetics of Plants,”, Vol. 3, I. K. Vasil, ed., Academic Press, New York, pp. 549–567.Google Scholar
  2. Hakman, I., and von Arnold, S., 1985, Plantlet regeneration through somatic embryogenesis in Picea abies (Norway spruce), J. Plant Physiol., 121:149–158.CrossRefGoogle Scholar
  3. Hakman, I., and Fowke, L. C., 1987, Somatic embryogenesis in Picea glauca (White spruce) and Picea mariana (Black spruce), Can. J. Bot., 65:656–659.CrossRefGoogle Scholar
  4. Halperin, W., 1986, Attainment and retention of morphogenic capacity in vitro, in: “Cell Culture and Somatic Cell Genetics of Plants,”, Vol. 3, I. K. Vasil, ed., Academic Press, New York, pp. 3–47.Google Scholar
  5. Joy, R. W., Patel, K. R., and Thorpe, T. A., 1988, Ascorbic acid enhancement of organogenesis in tobacco callus, Plant Cell Tiss. Org. Cult., 13:219–228.CrossRefGoogle Scholar
  6. Kartha, K. K., Fowke, L. C., Leung, N. L., Coswell, K. L., and Hakman, I., 1988, Induction of somatic embryos and plantlets from cryopreserved cell cultures of white spruce (Picea glauca), J. Plant Physiol., 132:529–539.CrossRefGoogle Scholar
  7. Lu, C.-Y., and Thorpe, T. A., 1987, Somatic embryogenesis and plantlet regeneration in cultured immature embryos of Picea glauca, J. Plant Physiol., 128:297–302.CrossRefGoogle Scholar
  8. Murashige, T., and Nakano, R., 1967, Chromosome complement as a determinant of the morphogenic potential of tobacco cells, Amer. J. Bot., 54:963–970.CrossRefGoogle Scholar
  9. Skoog, F., and Miller, C. O., 1957, Chemical regulation of growth and organ formation in plant tissues cultured in vitro, Symp. Soc. Exp. Biol., 11:118–131.PubMedGoogle Scholar
  10. Thorpe, T. A., and Meier, D. D., 1975, Effect of gibberellic acid on starch metabolism in tobacco callus cultures under shoot-forming conditions, Phytomorphology, 25:238–245.Google Scholar
  11. Thorpe, T. A., and Murashige, T., 1970, Some histochemical changes underlying shoot initiation in tobacco callus cultures, Can. J. Bot., 48:277–285.CrossRefGoogle Scholar
  12. Thorpe, T. A., 1988, Physiology of bud induction in conifers in vitro, in: “Genetic Manipulation of Woody Plants,” J. W. Hanover and D. E. Keathley, eds., Plenum Publishing, pp. 167–184.CrossRefGoogle Scholar
  13. von Arnold, S., and Eriksson, T., 1981, In vitro studies of adventitious shoot formation in Pinus contorta, Can. J. Bot., 59:870–874.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Richard W. JoyIV
    • 1
  • Trevor A. Thorpe
    • 1
  1. 1.Plant Physiology Research Group, Department of Biological SciencesUniversity of CalgaryCalgaryCanada

Personalised recommendations