Embryogenic Cultures of Tobacco Pollen as a Model System to Study Plant Rejuvenation
Formation of pollen in angiosperms takes place in the anthers, the male sexual organs. During normal male gametophytic development in vivo, each diploid microspore mother cell generates, through meiosis, four haploid microspores; after one or two mitosis and maturation, they will eventually give rise to mature pollen grains. However, in in vitro cultures of isolated anthers or pollen, pollen grains are able to follow a different, sporophytic developmental pathway, leading to the formation of embryos and haploid plants directly and asexually. Recent advances in the technique of isolated pollen culture in the model plant tobacco, allow the strict control of pollen development in both the sporophytic and the gametophytic direction.
KeywordsCytoplasmic Male Sterility Anther Culture Pollen Development Embryogenic Culture Anther Wall
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- Benito Moreno, R.M., Maqke, F., Hauser, M.-T., Alwen, A., and Heberle-Bors, E., 1988b, Sporophytes and male gametophytes from in vitro cultured, immature tobacco pollen, pp 137–142, in “Sexual Reproduction in Higher Plants”, M. Cresti, P. Gori, and E. Pacini, eds., Springer, New York Berlin Heidelberg.CrossRefGoogle Scholar