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Plant Aging pp 389-393 | Cite as

Embryogenic Cultures of Tobacco Pollen as a Model System to Study Plant Rejuvenation

  • Oscar Vicente
  • Dolores Garrido
  • Norbert Eller
  • Rosa M. Benito Moreno
  • Anna Alwen
  • Erwin Heberle-Bors
Part of the NATO ASI Series book series (NSSA, volume 186)

Abstract

Formation of pollen in angiosperms takes place in the anthers, the male sexual organs. During normal male gametophytic development in vivo, each diploid microspore mother cell generates, through meiosis, four haploid microspores; after one or two mitosis and maturation, they will eventually give rise to mature pollen grains. However, in in vitro cultures of isolated anthers or pollen, pollen grains are able to follow a different, sporophytic developmental pathway, leading to the formation of embryos and haploid plants directly and asexually. Recent advances in the technique of isolated pollen culture in the model plant tobacco, allow the strict control of pollen development in both the sporophytic and the gametophytic direction.

Keywords

Cytoplasmic Male Sterility Anther Culture Pollen Development Embryogenic Culture Anther Wall 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Benito Moreno, R.M., Macke, F., Alwen, A., and Heberle-Bors, E., 1988a, In situ seed production after pollination with in vitro matured, isolated pollen, Planta, 176:145–148.CrossRefGoogle Scholar
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  3. Heberle-Bors, E., 1989, Isolated pollen culture in tobacco: plant reproductive development in a nutshell. Sex. Plant Reprod., 2:1–10.CrossRefGoogle Scholar
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Oscar Vicente
    • 1
  • Dolores Garrido
    • 1
  • Norbert Eller
    • 1
  • Rosa M. Benito Moreno
    • 1
  • Anna Alwen
    • 1
  • Erwin Heberle-Bors
    • 1
  1. 1.Institut für Mikrobiologie und GenetikUniversität WienWienAustria

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