Plant Aging pp 383-387 | Cite as

Rejuvenation of Adult Specimens of Castanea Sativa Mill: Through In Vitro Micropropagation

  • José A. Feijó
  • M. Salomé S. Pais
Part of the NATO ASI Series book series (NSSA, volume 186)


Castanea sativa is a woody plant difficult to propagate by conventional methods. Cuttings are difficult to root, and, due to the almost complete autoincompatibility, the levels of heterozygoty are very high, thus compromising programs of propagation by seeds. Until recently some success was obtained on the micropropagation of adult plants of C.sativa × C.crenata hybrids (reviewed by Vieitez et al.,1986). However, as stressed by several authors trees and shrubs can only be selected for cloning when they are adult (cf. Bonga and Durzan,eds.,1982, and Pierik,1987). As a consequence, efficient methods of micropropagation of adult specimens are required if cloning of a specific tree is needed. In C.sativa different attempts of micropropagate adult material have failed systematically in the rooting step (Chevre, 1985; Mullins, 1987). The presence of rooting inhibitors in mature material may be one of the reasons of this failure, since this kind of compounds seems to be absent from juvenile and etiolated material (Gesto et al.,1977; Vieitez et al.,1987). Since C.sativa lacks other precise juvenility markers, the ability of rooting could therefore be considered as a rough rejuvenation marker. Recently a complete process of micropropagation of adult material of C.sativa has been described (Feijô and Pais,1988; Feijô and Pais,1989). This paper describes some results on this process related to a possible rejuvenation occurring in vitro.


Adult Specimen Culture Initiation Mature Material Juvenile Material Adult Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bonga, JM, Durzan, DJ, eds., 1982, Tissue Culture in Forestry, Martinus Nijhoff & W.Junk Publ.,The Hague, 1,420Google Scholar
  2. Bonga, JM, Durzan, DJ, eds., 1987, Cell and Tissue Culture in Forestry, vols. 1–3, Martinus Nijhoff Pub., DordrechtGoogle Scholar
  3. Chevre, AM, 1985, Recherches sur la multiplication vegetative in vitro chez le chaitaigner. These Doct., Univ. Bordeaux, BordeauxGoogle Scholar
  4. Gesto, MDV, Vasquez, A, Vieitez, E, 1977, “Rooting substances in water extracts of Castanea sativa and Salix viminalis”, Physiol. Plant., 40: 265, 268Google Scholar
  5. Gresshof, PM, Doy, CH, 1972, “Development and Differentiation of haploid Lycopersicum esculentum (tomato)” Planta, 17: 161, 170Google Scholar
  6. Feijo, JF, Pais, MS, 1988, “Micropropagaçäo de Castanea sativa Mill. a partir de gemas axilares de espécimes adultos”, Proc.Reuniao Soc.Port.Biotecnol., SPB, CoimbraGoogle Scholar
  7. Feijo, JF, Pais, MS, 1989, “In vitro propagation of adult trees of Castanea sativa Mill, through axillary bud culture”, Submitted Google Scholar
  8. Marques, CM, 1988, “Estabelecimento de Castanea sativa in vitro”, Relatorio de projecto, Fac. Ciências de Lisboa, Lisboa.Google Scholar
  9. Moncousin, J, 1982, Proc.5th Int. Cong.Plant Tissue Cell Culture, 5: 147,148Google Scholar
  10. Mullins, KV, 1987, “Micropropagation of chestnut (Castanea sativa Mill.)”, Acta Horticulturae, 212:525,530Google Scholar
  11. Pierik, 1987, In, Vitro culture of higher plants, Martinus Nijhoff Pub., DordrechtCrossRefGoogle Scholar
  12. Vieitez, AM, Vieitez, ML, Vieitez, E, 1986, “Chestnut (Castanea spp.)” in Biotechnology in Agriculture and Forestry, YPS Bajaj, ed., Springer-Verlag, Berlin, Vol.1 (Trees), 393, 414Google Scholar
  13. Vieitez, J, Kingston, DGI, Ballester, A, Vieitez, E, 1987, “Identification of two compounds correlated with lack of rooting capacity of chestnut cuttings”, Tree Physiol., 3: 247,255Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • José A. Feijó
    • 1
  • M. Salomé S. Pais
    • 1
  1. 1.Secçäo de Biologia Celular, Departamento de Biologia VegetalFaculdade de Ciências de Lisboa R, Ernesto de Vasconcelos, Ed. C2LisboaPortugal

Personalised recommendations