Cuticle Development in Dianthus Caryophyllus Plantlets
Cuticle development on “in vitro” developed leaves is important in order to prevent excessive water loss during acclimatization of “in vitro” micropropagated plants. Therefore quantitative and qualitative content of epicuticular wax on leaves of Dianthus caryophyllus has been studied from the “in vitro” juvenile stage to adult plants acclimatized and grown in greenhouse conditions.
The results show there are different cuticles all through their development. Thus “in vitro” developed leaves have less epicuticular wax than adult, greenhouse adapted plants, and the chemical composition was also different after acclimatization.
KeywordsCompound Class Cuticular Transpiration Irreversible Tissue Damage Excessive Water Loss Cuticle Development
Unable to display preview. Download preview PDF.
- Casares, A., Estrada, O., Astorga, R., and Rodríguez, R., 1987, Aplicaciones del cultivo de tejidos I. Saneamiento de clavel mediante cultivo de meristemos, Rev. Soc. Esp. Hort., 6.882: 8–10.Google Scholar
- George, E. F., and Sherrington, P. D., 1984, “Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories”, Exegetics Ltd., Eversley, Bas ingtoke.Google Scholar
- Krebs, K. G., Heusser, D., and Wimmer, H., 1969, Spray reagents, in: “Thin-Layer Chromatography. A Laboratory Handbook”, E. Stahl ed.. Springer Verlag, Berlin.Google Scholar
- Martin, J. T., and Juniper, B. E., 1970, “The Cuticles of Plants”, Edward Arnold Ltd., Edinburgh.Google Scholar
- Sutter, E., 1988, Stomatal and cuticular water loss from apple, cherry, and sweetgum plants after removal from in vitro culture,J. Amer. Soc. Hort. Sci. 113(2): 234–238.Google Scholar
- Yeoman, M. M., 1986, “Plant Cell Culture Technology. Botanical Monographs. Vol. 23”, J. H. Burnett, H. G. Baker, H. Beevers and F. R. Whatley ed. Blackwell Scientific Publications, Edinburgh.Google Scholar
- Ziv, M., 1986, In vitro hardening and acclimatization of tissue culture plants, in: “Plant Tissue Culture and its Agricultural Applications”, L. A. Withers and P. G. Alderson ed., Butterworths, Cambridge.Google Scholar
- Zweig, G., and Sherma, J., 1972, “Handbook of Chromatography. Vol II”, CRC Press, Cleveland.Google Scholar