Abstract
Cuticle development on “in vitro” developed leaves is important in order to prevent excessive water loss during acclimatization of “in vitro” micropropagated plants. Therefore quantitative and qualitative content of epicuticular wax on leaves of Dianthus caryophyllus has been studied from the “in vitro” juvenile stage to adult plants acclimatized and grown in greenhouse conditions.
The results show there are different cuticles all through their development. Thus “in vitro” developed leaves have less epicuticular wax than adult, greenhouse adapted plants, and the chemical composition was also different after acclimatization.
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References
Bengtson, C., Larsson, S., and Liljenberg, C., 1978, Effects of water stress on cuticular transpiration rate and amount and composition of epicuticular wax in seedlings of six oat varieties, Physiol. Plant., 44: 319–324.
Casares, A., Estrada, O., Astorga, R., and Rodríguez, R., 1987, Aplicaciones del cultivo de tejidos I. Saneamiento de clavel mediante cultivo de meristemos, Rev. Soc. Esp. Hort., 6.882: 8–10.
George, E. F., and Sherrington, P. D., 1984, “Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories”, Exegetics Ltd., Eversley, Bas ingtoke.
Krebs, K. G., Heusser, D., and Wimmer, H., 1969, Spray reagents, in: “Thin-Layer Chromatography. A Laboratory Handbook”, E. Stahl ed.. Springer Verlag, Berlin.
Larsson, S., and Svenningsson, M., 1986, Cuticular transpiration and epicuticular lipids of primary leaves of barley (Hordeum vulgare), Physiol. Plant., 68: 13–19.
Martin, J. T., and Juniper, B. E., 1970, “The Cuticles of Plants”, Edward Arnold Ltd., Edinburgh.
Murashige, T., and Skoog, F., 1962, A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant., 15: 473–497.
Sutter, E., 1988, Stomatal and cuticular water loss from apple, cherry, and sweetgum plants after removal from in vitro culture,J. Amer. Soc. Hort. Sci. 113(2): 234–238.
Yeoman, M. M., 1986, “Plant Cell Culture Technology. Botanical Monographs. Vol. 23”, J. H. Burnett, H. G. Baker, H. Beevers and F. R. Whatley ed. Blackwell Scientific Publications, Edinburgh.
Ziv, M., 1986, In vitro hardening and acclimatization of tissue culture plants, in: “Plant Tissue Culture and its Agricultural Applications”, L. A. Withers and P. G. Alderson ed., Butterworths, Cambridge.
Zweig, G., and Sherma, J., 1972, “Handbook of Chromatography. Vol II”, CRC Press, Cleveland.
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© 1990 Plenum Press, New York
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Fal, M.A., Bernad, P., Obeso, R., Tamés, R.S. (1990). Cuticle Development in Dianthus Caryophyllus Plantlets. In: Rodríguez, R., Tamés, R.S., Durzan, D.J. (eds) Plant Aging. NATO ASI Series, vol 186. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5760-5_47
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DOI: https://doi.org/10.1007/978-1-4684-5760-5_47
Publisher Name: Springer, Boston, MA
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