Plant Aging pp 361-365 | Cite as

Segmentation Effect of Immature Spike on Triticale Calli Induction

  • H. Guedes-Pinto
  • O. Pinto-Carnide
  • F. Leal
Part of the NATO ASI Series book series (NSSA, volume 186)


Calli induction is a stepwise process to obtain regenerated plants via somatic cells, cell suspension, genetic transformation, mutagenesis, etc. (see review of Maddock, 1985; Vasil, 1987 and Conger et al.,1988). Immature spikes proved to be a fine expiant to the induction of calli in triticale (X Triticosecale) and other cereals (Ozias-Akins and Vasil, 1982; Rangan and Vasil, 1983; Eapen and Rao, 1985; Fedak, 1985; Sun and Zhu, 1987).


Somatic Embryogenesis Plant Regeneration Calli Induction Spike Length Segmentation Effect 


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  1. Conger, B.W., Trigiano, R.N. and Gray, D.J., 1988. Cell Culture of the Poaceae (Gramineae). In: Plant Cell Biotechnology. Ed. M. Pais, F. Mavituna & J.M. Novais. NATO A5I Series, Cell Biology (18): 49–61.CrossRefGoogle Scholar
  2. Conger, B.W., Hanning, G.E., Gray, D.J. and McDaniel, K., 1983. Direct embryogenesis from mesophyll cells of orchardgrass. Science, 221: 850–851PubMedCrossRefGoogle Scholar
  3. Eapen, S. and Rao, P.S., 1985. Plant regeneration from immature inflorescence callus culture of wheat, rye and triticale. Euphytica, 34: 153–159.CrossRefGoogle Scholar
  4. Fedak, G.,1985. Propagation of intergeneric hybrids of Triticeae through callus culture of immature inflorescences. Z. Pflanzenzuchtunq, 94 (1): 1–7.Google Scholar
  5. Guedes-Pinto, H. and Pinto-Carnide, O., 1987. Plantlets regeneration from in vitro culture of 6x-triticale immature spikes. Ciência Biolóqica (Supl.), 12(5A): 208.Google Scholar
  6. Guedes-Pinto, H., Pinto-Carnide, O. and Alpoim, F., 1989. Calli induction from Triticale Immature Spikes. Vortrage für Pflanzenzuchtunq, Heft 15–1: 7–14.Google Scholar
  7. Maddock, S.E., 1985. Cell culture, somatic embryogenesis and plant regeneration in wheat, barley, oats, rye and triticale. In Cereal Tissue and Cell Culture, Ed. S. U.J. Bright and M. G.K. Jones, Martinus Nighoff/Dr. W. Junk Publ., Dordrecht: 131–174.CrossRefGoogle Scholar
  8. Ozias-Akins, P. and Vasil, J.K., 1982. Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (Uheat), evidence for somatic embryogenesis. Protoplasma,110; 95–105.CrossRefGoogle Scholar
  9. Rangan, T.S. and Vasil, I.K., 1983. Somatic Embryogenesis and Plant Regeneration in Tissue Culture of Panicum miliaceum L. and Panicum miliare Lamk.. Z.Pflanzenphysiol, 109: 49–53.Google Scholar
  10. Saalbach, G. and Koblitz, H.,1978. Attempts to initiate callus formation from barley leaves. Plant Science Letters, 13:165–169.CrossRefGoogle Scholar
  11. Sun, J.S. and Zhu, Z.Q.,1987. Culture of expiants from immature inflorescence of octoploid triticale (Abs.). Uheat, Barley and Triticale Abstracts, Feb., 4(1): 89.Google Scholar
  12. Vasil, I.K., 1988. Developing Cell and Tissue Culture Systems for the Improvement of Cereal and Grass Crops. J. Plant Physiol.,128: 193–218.CrossRefGoogle Scholar
  13. Vasil, V. and Vasil, J.K., 1984. Induction and maintenance of embryo-genetic callus cultures of Gramineae. In Cell Culture and Somatic Cell Genetics of Plants, Vol. 1, Ed. J.K. Vasil, Academic Press, Inc., Orlando: 36–42.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • H. Guedes-Pinto
    • 1
  • O. Pinto-Carnide
    • 1
  • F. Leal
    • 1
  1. 1.Genetics and Plant Breeding DivisionUniversity of Trás-os-Montes e Alto DouroVila Real CodexPortugal

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