Plant Aging pp 277-284 | Cite as

Polyamines and Aging: Effect of Polyamine Biosynthetic Inhibitors on Plant Regeneration in Maize Callus Cultured in Vitro

  • A. F. Tiburcio
  • X. Figueras
  • I. Claparols
  • M. Santos
  • J. Ma. Torné
Part of the NATO ASI Series book series (NSSA, volume 186)


Culture age is an important factor in expressing the genetic potential of plant cells and tissues in vitro, since it is well known that many cultures lose their morphogenetic capacity as they aged (Vasil et al., 1984). Although the precise biochemical and molecular mechanisms underlying the gradual loss of totipotency are not known, some compounds such as polyamines (PAs) are involved in these phenomena. Thus, the diamine putrescine (Put), the triamine spermidine (Spd), the tetraamine spermine (Spm), and their biosynthetic enzyme arginine decarboxylase (ADC) are biochemical markers of both plant cell aging and plant cell differentiation. In senescing leaves of cereals incubated in darkness both the ADC activity and the endogenous PA levels progressively decrease, while exogenous application of PAs, especially Spd and Spm, inhibits or retards the symptoms of senescence (Kaur-Sawhhey et al., 1979; 1982). On the other hand, using tobacco thin cell layer cultures (TCL; Tran Thanh Van, 1973) we have demonstrated that Spd is a marker of floral differentiation (Tiburcio et al., 1987; 1988; Kaur-Sawhney et al., 1988), while the Put formed via ADC is a marker of root differentiation (Tiburcio et al., 1989a).


Leaf Senescence Polyamine Metabolism Anti Senescence Effect Diamine Putrescine Maize Callus 
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  1. Balestreri, E., Cioni, P., Romagnoli, A., Bernini, S., Fissi, A., Felicioli, R., 1987, Mechanism of polyamine inhibition of a leaf protease, Arch. Biochem. Biophys., 255: 460.PubMedCrossRefGoogle Scholar
  2. Bradford, M.M., 1976, A rapid method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72:248.PubMedCrossRefGoogle Scholar
  3. Carbonell, J., and Navarro, J.L., 1989, Correlation of spermine level with ovary senescence and fruit set and development in Pisum sativum, Planta, (in press).Google Scholar
  4. Flores, H.E., and Galston, A.W., 1982, Polyamines and plant stress: Activation of putrescine biosynthesis by osmotic shock, Science, 217:1259.PubMedCrossRefGoogle Scholar
  5. Kallio, A., McCann, P.P., and Bey, P., 1981, DL-alpha-difluoromethylarginine: A potent enzyme-activated irreversible inhibitor of bacterial arginine decarboxylases, Biochemistry, 20:3163.PubMedCrossRefGoogle Scholar
  6. Kaur-Sawhney, R., and Galston, A.W., 1979, Interaction of polyamines and light on biochemical processes involved in leaf senescence, Plant Cell Environ., 2:189.CrossRefGoogle Scholar
  7. Kaur-Sawhney, R., Shih, L.M., Flores, H.E., and Galston, A.W., 1982, Relation of polyamine synthesis and titer to aging and senescence in oat leaves, Plant Physiol., 69:405.PubMedCrossRefGoogle Scholar
  8. Kaur-Sawhney, R., Shih, L.M., Cegielska, T., and Galston, A.W., 1982, Inhibition of protease activity by polyamines. Relevance for control of leaf senescence, FEBS Lett., 145:345.Google Scholar
  9. Kaur-Sawhney, R., Shekhawat, N.S., and Galston, A.W., 1985, Polyamine levels as related to growth, differentiation and senescence in protoplast-cultures of Vigna aconitifolia and Avena sativa, Plant Growth Regul. 3:329.PubMedCrossRefGoogle Scholar
  10. Kaur-Sawhney, R., Tiburcio, A.F., and Galston, A.W., 1988, Spermidine and flower bud differentiation in thin layer tobacco tissue cultures, Planta, 173:282.CrossRefGoogle Scholar
  11. Metcalf, B.W., Bey, P., Danzin, C., Jung, M.J., Casara, P., and Vevert, J.P., 1978, Catalytic irreversible inhibition of mammalian ornithine decarboxylase (EC by substrate and product analogues, J. Amer. Chem. Soc., 100:2551.CrossRefGoogle Scholar
  12. Slocum, R.D., and Galston, A.W., 1987, Inhibition of polyamine biosynthesis in plants and plant pathogenic fungi, in: “Inhibition of Polyamine Metabolism: Biological Significance and Basis for New Therapies”, P.P. McCann, A.E. Pegg and A. Sjoerdsma, eds., Academic Press, New York.Google Scholar
  13. Thomas, H., and Stoddart, J.L., 1980, Leaf senescence, Annu, Rev. Plant Physiol., 31:83.CrossRefGoogle Scholar
  14. Tiburcio, A.F., Kaur-Sawhney, R., Ingersoll, R., and Galston, A.W., 1985, Correlation between polyamines and pyrrolidine alkaloids in developing tobacco callus, Plant Physiol., 78:323.PubMedCrossRefGoogle Scholar
  15. Tiburcio, A.F., Masdéu, M.A., Dumortier, F.M., and Galston, A.W., 1986a, Polyamine metabolism and osmotic stress: I. Relation to protoplast viability, Plant Physiol., 82:369.CrossRefGoogle Scholar
  16. Tiburcio, A.F., Kaur-Sawhney, R., and Galston, A.W., 1986b, Polyamine metabolism and osmotic stress: Improvement of oat protoplast by an inhibitor of putrescine biosynthesis, Plant Physiol., 82:375.CrossRefGoogle Scholar
  17. Tiburcio, A.F., Kaur-Sawhney, R., and Galston, A.W., 1987, Regulation by polyamines of plant tissue culture development, in: “Advances in the Chemical Manipulation of Plant Tissue Cultures”, M.B. Jackson, S.H. Mantell and J. Blake, eds., British Plant Growth Regulator Group, Bristol.Google Scholar
  18. Tiburcio, A.F., Kaur-Sawhney, R., and Galston, A.W., 1988, Polyamine biosynthesis during vegetative and floral bud differentiation in thin layer tobacco tissue cultures, Plant Cell Physiol., 29:1241.Google Scholar
  19. Tiburcio, A.F., Gendy, C.A., and Tran Thanh Van, K., 1989a, Morphogenesis in tobacco subepidermal cells: putrescine as marker of root differentiation, Plant Cell Tiss. Org. Cult., (in press).Google Scholar
  20. Tiburcio, A.F., Kaur-Sawhney, R., and Galston, A.W., 1989b, Polyamine metabolism, in: “The Biochemistry of Plants. A Comprehensive Treatise,” B.J. Miflin, ed., Academic Press, New York, (in press).Google Scholar
  21. Torné, J.M., Santos, M.A., and Blanco, J.L., 1984, Methods of obtaining maize totipotent tissues. II. Atrophic tissue culture, Plant Sci. Lett., 33:317.CrossRefGoogle Scholar
  22. Tran Thanh Van, K., 1973, Direct flower neoformation from superficial tissues of small expiant of Nicotiana tabacum L., Planta, 115:87.CrossRefGoogle Scholar
  23. Vasil, V., Vasil, I.K., and Chin-yi Lu, 1984, Somatic embryogenesis in long-term callus cultures of Zea mays L. (Gramineae), Am. J. Bot., 71:158.CrossRefGoogle Scholar
  24. Wyss-Benz, M., Streit, L., and Ebert, E., 1988, Hydroxycinnamoyl amides in stem expiants from flowering and non-flowering Nicotiana tabacum, Physiol. Plant., 74:294.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • A. F. Tiburcio
    • 1
  • X. Figueras
    • 1
  • I. Claparols
  • M. Santos
    • 2
  • J. Ma. Torné
    • 2
  1. 1.Laboratori Fisiologia Vegetal, Facultat de FarmàciaUniversitat BarcelonaBarcelonaSpain
  2. 2.C.I.D.-C.S.I.C.BarcelonaSpain

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