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Plant Aging pp 277-284 | Cite as

Polyamines and Aging: Effect of Polyamine Biosynthetic Inhibitors on Plant Regeneration in Maize Callus Cultured in Vitro

  • A. F. Tiburcio
  • X. Figueras
  • I. Claparols
  • M. Santos
  • J. Ma. Torné
Part of the NATO ASI Series book series (NSSA, volume 186)

Abstract

Culture age is an important factor in expressing the genetic potential of plant cells and tissues in vitro, since it is well known that many cultures lose their morphogenetic capacity as they aged (Vasil et al., 1984). Although the precise biochemical and molecular mechanisms underlying the gradual loss of totipotency are not known, some compounds such as polyamines (PAs) are involved in these phenomena. Thus, the diamine putrescine (Put), the triamine spermidine (Spd), the tetraamine spermine (Spm), and their biosynthetic enzyme arginine decarboxylase (ADC) are biochemical markers of both plant cell aging and plant cell differentiation. In senescing leaves of cereals incubated in darkness both the ADC activity and the endogenous PA levels progressively decrease, while exogenous application of PAs, especially Spd and Spm, inhibits or retards the symptoms of senescence (Kaur-Sawhhey et al., 1979; 1982). On the other hand, using tobacco thin cell layer cultures (TCL; Tran Thanh Van, 1973) we have demonstrated that Spd is a marker of floral differentiation (Tiburcio et al., 1987; 1988; Kaur-Sawhney et al., 1988), while the Put formed via ADC is a marker of root differentiation (Tiburcio et al., 1989a).

Keywords

Leaf Senescence Polyamine Metabolism Anti Senescence Effect Diamine Putrescine Maize Callus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • A. F. Tiburcio
    • 1
  • X. Figueras
    • 1
  • I. Claparols
  • M. Santos
    • 2
  • J. Ma. Torné
    • 2
  1. 1.Laboratori Fisiologia Vegetal, Facultat de FarmàciaUniversitat BarcelonaBarcelonaSpain
  2. 2.C.I.D.-C.S.I.C.BarcelonaSpain

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