Plant Aging pp 19-25 | Cite as

Adult vs. Juvenile Explants: Directed Totipotency

  • D. J. Durzan
Part of the NATO ASI Series book series (NSSA, volume 186)


The exploitation of stages of the life cycle of a woody perennial through cell and tissue culture is examined in terms of explant selection and discrete developmental outcomes. For mass propagation, explants from adult trees require rejuvenation, dedifferentiation, induction and redifferentiation. These processes traverse phases of the life cycle and involve cells having cell-line replicator activity. The components of the rejuvenation process, replicator monitoring and the concept of directed totipotency are illustrated with Prunus sp. and with some conifers. The result leads to some general notions for diagnostic reasoning in ontogeny under artificial conditions and different genetic backgrounds.


Somatic Embryogenesis Replicator Activity Diagnostic Reasoning Woody Perennial Restriction Fragment Length Polymorphism Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bochner, B.R., 1989, Sleuthing out bacterial identities, J. Genet., 65:45–54.Google Scholar
  2. Bonga, J.M., 1982, Vegetative propagation in relation to juvenility, maturity and rejuvenation, in: “Tissue Culture in Forestry,” J.M. Bonga and DJ. Durzan, eds., Martinus Nijhoff, Dordrecht.Google Scholar
  3. Bonga, J.M., von Aderkas, P., and James, D., 1988, Potential application of haploid cultures of tree species, in: “Genetic Manipulation of Woody Plants,” J.W. Hanover and D.E. Keathley, eds., Plenum Press, N.Y., pp. 57–77.CrossRefGoogle Scholar
  4. Brink, R.A., 1962, Phase change in higher plants and somatic cell heredity, Q. Rev. Biol., 37:1–22.CrossRefGoogle Scholar
  5. Dawkins, R., 1982, “The Extended Phenotype,” WH Freeman Co., San Francisco, CA.Google Scholar
  6. Durzan, D.J., 1982, Somatic embryogenesis and sphaeroblasts in conifer cell suspensions. Proc. 5th Intl. Cong. Plant Cell Tissue Culture, July 11–16, 1982, Tokyo, Japan, 113–114.Google Scholar
  7. Durzan, D.J., 1984a, Special problems: Adult vs. juvenile expiants, Chap. 17, in: “Handbook of Plant Cell Culture, Crop Species,” W.R. Sharp, D.A. Evans, P.V. Ammirato, and Y. Yamada, eds., MacMillan Publishing Co., N.Y. 2:471–503.Google Scholar
  8. Durzan, D.J., 1984b, Potential for genetic manipulation of forest trees: Totipotency, somaclonal aberration, and trueness to type, in: “Proceedings International Symposium on Recent Advances in Forest Biotechnology,” July 10–13, 1984, Michigan Biotechnology Institute, Traverse City, Michigan, pp. 104–125.Google Scholar
  9. Durzan, D.J., 1987a, Plant growth regulators in cell and tissue culture of woody perennials, Plant Growth Regulation, 6:95–112.CrossRefGoogle Scholar
  10. Durzan, D.J., 1987b, Physiological states and metabolic phenotypes in embryonic development, in: “Cell and Tissue Culture in Forestry,” J.M. Bonga and D.J. Durzan, eds., Martinus Nijhoff/Dr. W. Junk, Dordrecht. Vol. 2, pp. 405–439.CrossRefGoogle Scholar
  11. Durzan, D.J., 1988a, Somatic polyembryogenesis and plantlet regeneration in selected tree crops, Biotech. Gen. Eng. Revs. 6:339–376.Google Scholar
  12. Durzan, D.J., 1988b, Process control in somatic polyembryogenesis, in: “Molecular Genetics of Forest Trees,” J.-E. Hällgren, ed., Frans Kempe Symp., Umea, Sweden, pp. 147–186.Google Scholar
  13. Durzan, D.J., 1988c, Applications of cell and tissue culture in tree improvement, in: “Applications of Plant Cell and Tissue Culture,” Ciba Foundation Symp. 137, John Wiley & Sons, N.Y. pp. 36–49.Google Scholar
  14. Durzan, D.J., 1990a, Performance criteria in response surfaces for metabolic phenotypes of clonally propagated woody perennials, in: “Application of Plant Biotechnology in Forestry,” V. Dhawan, ed., Plenum Press, N.Y. (in press).Google Scholar
  15. Durzan, D.J., 1990b, Molecular phenogenetics as an aid to fruit breeding, Acta Hort. (in press).Google Scholar
  16. Durzan, D.J. and Durzan, P.E., 1989, Future technologies: Model-reference control systems for the scale-up of embryogenesis and polyembryogenesis in cell suspension cultures, in: “Micropropagation,” P. Debergh and R. Zimmerman, eds., Martinus Nijhoff, Dordrecht. (in press).Google Scholar
  17. Glock, H. and Gregorius, H.-R., 1984, Differentiation — A consequence of ideotype-environment interaction, BioSystems, 17:23–34.PubMedCrossRefGoogle Scholar
  18. Gupta, P.K. and Durzan, D.J., 1987, In vitro establishment and multiplication of Juvenile and mature Douglas-fir and sugar pine, Symposium: “In Vitro Problems Related to Mass Propagation of Horticultural Plants,” September 16–20, 1985, Gembloux, Belgium, Acta Hort., 212:483–487.Google Scholar
  19. Hansche, P.E., 1983, Response to selection, in: “Methods in Fruit Breeding,” J.N. Moore and J. Janick, eds., Purdue Univ. Press, West Lafayette, Indiana pp. 154–171.Google Scholar
  20. Hansen, K.C. and Durzan, D.J., 1986, Somatic embryogenesis and morphogenesis in Prunus cerasus, Proc. XXII Intl. Hort. Cong., August 10–18, 1986, University of California, Davis, CA. Abstr. No. 1130.Google Scholar
  21. Mandelbrot, B.B., 1982, “The Fractal Geometry of Nature,” W.H. Freeman, N.Y.Google Scholar
  22. Steward, F.C., 1968, “Growth and Organization in Plants,” Addison-Wesley Publ. Co., Reading, Mass.Google Scholar
  23. Tanksley, S.D., Young, N.D., Paterson, A.H., and Bonierbale, M.W., 1989, RFCP mapping in plant breeding: New tools for an old science, Bio/Tech., 7:257–264.CrossRefGoogle Scholar
  24. Visser, J., 1976, A comparison of apple and pear seedlings with reference to the juvenile period, II. Mode of inheritance, Euphytica, 25:334–342.Google Scholar
  25. Wright, S., 1968, “Evolution and the Genetics of Populations,” Vol. 1, Univ. Chicago Press.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • D. J. Durzan
    • 1
  1. 1.Department of Environmental HorticultureUniversity of CaliforniaDavisUSA

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