Plant Aging pp 19-25 | Cite as

Adult vs. Juvenile Explants: Directed Totipotency

  • D. J. Durzan
Part of the NATO ASI Series book series (NSSA, volume 186)

Abstract

The exploitation of stages of the life cycle of a woody perennial through cell and tissue culture is examined in terms of explant selection and discrete developmental outcomes. For mass propagation, explants from adult trees require rejuvenation, dedifferentiation, induction and redifferentiation. These processes traverse phases of the life cycle and involve cells having cell-line replicator activity. The components of the rejuvenation process, replicator monitoring and the concept of directed totipotency are illustrated with Prunus sp. and with some conifers. The result leads to some general notions for diagnostic reasoning in ontogeny under artificial conditions and different genetic backgrounds.

Keywords

Sugar Agarose Bromide Macromolecule Ethidium 

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • D. J. Durzan
    • 1
  1. 1.Department of Environmental HorticultureUniversity of CaliforniaDavisUSA

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