Plant Aging pp 215-224 | Cite as

An Experimental Model for the Analysis of Plant/Cell Differentiation: Thin Cell Layer Concept, Strategy, Methods, Records and Potential

  • Kiem Tran Thanh Van
  • Luc Richard
  • Cyrille Amin Gendy
Part of the NATO ASI Series book series (NSSA, volume 186)


Plant differentiation including morphogenesis, growth, development and plant senescense result from complex interaction between different organs, tissues and cells. The analysis at the molecular level of the mechanisms of differentiation is difficult due to the lack of knowledge on i) the nature of the morphogenetic signal, ii) the perception and transduction mechanisms and iii) the localization of target cells and of responsive cells.


Responsive Cell Thin Cell Layer Inter Organ Network Morphogenetic Signal Morphogenetic Program 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Tran Thanh Van M., 1973, Direct flower neoformation from superficial tissue of small expiant of Nicotiana tabacum L. Planta 115:87.CrossRefGoogle Scholar
  2. Tran Thanh Van M., Thi Dien N., and Chlyah A., 1974, Regulation of organogenesis in small expiants of superficial tissue of Nicotiana tabacum L. Planta 119:149.CrossRefGoogle Scholar
  3. Tran Thanh Van M., 1980, Thin cell layers: Control of morphogeneis by inherent factors and exogenously applied factors. Int. Rev. Cytol. 11 A: 175 Academic Press.Google Scholar
  4. Albersheim P., Darvill A. G., McNeil M., Valent B. S., Sharp J. K., Nothnagel E. A., Davis K. R., Yamazaki N., Gollin D. J., York W. S., Dudman W. F., Darvill J. E., and Dell A., 1983, Oligosaccharins, naturally occurring carbohydrates with biological regulatory functions. In Structure and Function of Plant Genomes, eds. O. Ciferri and L. Dure III, pp. 293–312, Plenum N. Y.Google Scholar
  5. Tran Thanh Van K., Toubart P., Cousson A., Darvill A. G., Gollin D. J., Chelf P., and Albersheim P., 1985a, Manipulation of the morphogenetic pathway of tobacco expiants by oligosaccharins. Nature 314:615.CrossRefGoogle Scholar
  6. Tran Thanh Van K., Trinh H. T., 1985b, Organogenesis and cell differentiation in in vitro and in vivo systems. In Handbook of plant cell culture, eds. Evans, P. Ammirato, MacMilland N. Y.Google Scholar
  7. Tran Thanh Van K., 1980b, Control of morphogenesis or what shapes a group cells ? in Plant tissue culture, ed. Fiechter A., Springer Verlag, Berlin/Heidelberg/New York.Google Scholar
  8. Tran Thanh Van K., 1981, Control of Morphogeneis. Ann. Rev. Plant. Physiol. 32: 291.CrossRefGoogle Scholar
  9. Mutafstchiev S., Cousson A., and Tran Thanh Van K., 1987, Modulation of Cell growth and differentiation by pH and oligosaccharides. In British Plant Growth Regulator Group, Monograph 16. Advances in the Chemical Manipulation of Plant Tissue Cultures.Google Scholar
  10. Richard L., 1987, Etude de l’influence des oligosaccharides pariétaux sur la croissance et l’Organogenese. Mise au point de methodes d’isolement et de culture d’assises cellulaires et de protoplastes de sous-épiderme de Nicotiana tabacum L. Université P. et M. Curie. DEA de Biologie et Physiologie végétales.Google Scholar
  11. Thorpe T., Tran Thanh Van K., Gaspar T., 1978, Isoperoxidases in Epidermal Layers of Tobacco and Changes during Organ Formation in Vitro Physiol Plant. 44:388.CrossRefGoogle Scholar
  12. Kay L. E., and Basile D. V., 1987, Specific Peroxydase isoenzymes are correlated with organogenesis. Plant Physiol., 84:89.CrossRefGoogle Scholar
  13. Tiburcio A. F., Kaur-Sawhney R., and Gaslton A., 1988, Polyanime Biosynthesis during Vegetative and Floral Bud Differentiation in Thin Layer Tobacco Tissue Cultures. Plant Cell Physiol. 29(7): 1241.Google Scholar
  14. Tiburcio A. F., Gendy C. A., and Tran Thanh Van K., 1989, Morphogenesis in tobacco subepidermal cells: putrescine as marker of root differentiation. Plant cell tissue and organ culture (in press).Google Scholar
  15. Appelbaum A., Canellakis Z. N., Applewhite Ph. B., R-Kaur-Sawhney R., and Gaston A. W., 1988, Binding of Spermidine to a unique Protein in Thin Layer Tobacco Tissue Culture. Plant Physiol. 88:996.CrossRefGoogle Scholar
  16. Meeks Wagner D., Dennis E. S., Tran Thanh Van K., and Peacock W. J., 1989, Tobacco Genes Expressed during in Vitro Floral Initiation and Their Expression during Normal Plant Development. The Plant Cell, 1:25.PubMedCrossRefGoogle Scholar
  17. Ammirato Ph. V., 1987, Speeding Transgenic Plants. Biotechnoloy 5:1015.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Kiem Tran Thanh Van
    • 1
  • Luc Richard
    • 1
  • Cyrille Amin Gendy
    • 1
  1. 1.Laboratoire de Physiologie VégétaleCNRSGif sur YvetteFrance

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