Study of Gene Expression During in Vitro Culture of Tobacco thin Cell Layers by two-Dimensional Electrophoresis of Proteins
Compared to animal systems, plant systems have tremendous potential for organ regeneration, embryogenesis from somatic cells, pollen grains, and ovules, for early sexual reproduction in in vivo conditions and in vitro conditions. Advanced progress already made in genetic manipulation technology would have led to important plant improvement if its feasibility was not limited to a small number of species. This limit is due to the difficulties in controlling in vitro plant regeneration via protoplasts, cells, tissues and/or organs culture of recalcitrant species such as monocotyledons, leguminous, and woody species which also have great economic importance. It is generally observed in recalcitrant species that regeneration of organs can be induced only during the juvenile phase. For example, mature tissues of woody plants lose their ability to differentiate organs in vitro. It is important to understand the phenomenon of maturation in recalcitrant species as it is important to understand the mechanisms of organ regeneration in recalcitrant, and nonrecalcitrant species. Progress is slow, because of the lack of regeneration /maturation mutants and because of the complexity of the eucaryotic genome. In species such as Arabidopsis for which the genome size is relatively reduced and for which several mutants are available, it still lacks developmental and regeneration mutants. There exist mutants with altered flower shape; however, these mutations are not suitable for studying the control of organogenesis.
KeywordsLarge Subunit Indolebutyric Acid Thin Cell Layer Great Economic Importance Eucaryotic Genome
two-dimensional polyacrylamide gel electrophoresis
ribulose bispohosphate carboxylase/oxygenase
large subunit of rubisco
thin cell layer
- d0, dn
day 0, day n
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