Plant Aging pp 207-213 | Cite as

Study of Gene Expression During in Vitro Culture of Tobacco thin Cell Layers by two-Dimensional Electrophoresis of Proteins

  • Kiêm Tran Thanh Van
  • Michel Zivy
  • Alain Cousson
  • Hervé Thiellement
Part of the NATO ASI Series book series (NSSA, volume 186)


Compared to animal systems, plant systems have tremendous potential for organ regeneration, embryogenesis from somatic cells, pollen grains, and ovules, for early sexual reproduction in in vivo conditions and in vitro conditions. Advanced progress already made in genetic manipulation technology would have led to important plant improvement if its feasibility was not limited to a small number of species. This limit is due to the difficulties in controlling in vitro plant regeneration via protoplasts, cells, tissues and/or organs culture of recalcitrant species such as monocotyledons, leguminous, and woody species which also have great economic importance. It is generally observed in recalcitrant species that regeneration of organs can be induced only during the juvenile phase. For example, mature tissues of woody plants lose their ability to differentiate organs in vitro. It is important to understand the phenomenon of maturation in recalcitrant species as it is important to understand the mechanisms of organ regeneration in recalcitrant, and nonrecalcitrant species. Progress is slow, because of the lack of regeneration /maturation mutants and because of the complexity of the eucaryotic genome. In species such as Arabidopsis for which the genome size is relatively reduced and for which several mutants are available, it still lacks developmental and regeneration mutants. There exist mutants with altered flower shape; however, these mutations are not suitable for studying the control of organogenesis.


Large Subunit Indolebutyric Acid Thin Cell Layer Great Economic Importance Eucaryotic Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



two-dimensional polyacrylamide gel electrophoresis


ribulose bispohosphate carboxylase/oxygenase


large subunit of rubisco


thin cell layer

d0, dn

day 0, day n


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Cannon G., Tran Thanh Van K., Heinhorst S., Trinh H., and Weissbach A., 1989, Plastici DNA in tobacco hypohaploïd plants. Plant Physiology. 90:390.PubMedCrossRefGoogle Scholar
  2. Colas des Francs C., and Thiellement H., 1985, Chromosomal localization of structural genes and regulatores in wheat by 2D electrophoresis of ditelosomic lines. Theor. Appl. Genet., 71:31.CrossRefGoogle Scholar
  3. Cousson A., Toubart P.,Tran Thanh Van K., 1989, Control of morphogenesis in tobaco thin cell layer by culture medium pH. Ca. Journ. of Bot.(in press).Google Scholar
  4. Damerval C, de Vienne D., Zivy M., and Thiellement H., 1986, Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat seedling proteins. Electrophoresis, 7:52.CrossRefGoogle Scholar
  5. Granier F. and de Vienne D., 1986, Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets. Anal. Biochem., 155:45.PubMedCrossRefGoogle Scholar
  6. Kay L. E. and Basile D. V., 1987, Specific Peroxydase isoenzymes are correlated with organogenesis. Plant Physiol., 84:99.PubMedCrossRefGoogle Scholar
  7. Meeks-Wagner D.R., Dennis E.S., Tran Thanh Van K., and Peacock W.J., 1989, Tobacco genes expressed in in vitro floral initiation and their expression during normal plant development. The PlantCell., 1: 25.Google Scholar
  8. Mulin M. and Tran Thanh Van K., 1989, Obtention in vitro of flowers from thin epidermal cell layers of partial somatic hybrid between Petunia hybrida. Plant Science., 62:113.CrossRefGoogle Scholar
  9. Mutafstchiev S., Cousson A., Tran Thanh Van K., 1988, Effect of chemical factors in the regulation of cell growth, in “Monograph 16 British group on plant growth regulators,Google Scholar
  10. M. Jackson. Oakley B. B., Kirsh D. R., and Morris N. R., 1980, A simplified silver stain for detecting proteins in Polyacrylamide gels. Anal.Bipchem., 105:361.Google Scholar
  11. O’Farrell P. H., 1975, High resolution two-dimensional electrophoresis of proteins.J. Biol. Chem., 250:4007.PubMedGoogle Scholar
  12. Sung Z.R. and Okimoto R., 1983, Coordinate gene expression during somatic embryogenesis in carrots. Proc. Natl. Acad. Sci. USA. 80: 2661PubMedCrossRefGoogle Scholar
  13. Tran Thanh Van K.,1973, In vitro control of de novo flower, bud, root, and callus differentiation from excised epidermal tissue. Nature, 246:44.CrossRefGoogle Scholar
  14. Tran Thanh Van K., 1980, Thin cell layers: control of morphogenesis by inherent factors and exogenously applied factors, in Int.Rev. Cytol., 11A, Vasil I., ed., Academic Press, 175.Google Scholar
  15. Tran Thanh Van K., 1981, Control of morphogenesis. Ann. Rev. PlantPhysiol., 32:291.CrossRefGoogle Scholar
  16. Tran Thanh Van K. and Marcotte J.L., 1981, Influence of the nature of cytokinins on the control of organogenesis in tobacco thin cell layer. Proceedings of XIII Int. Botanical Congress, Sydney, Australia.Google Scholar
  17. Tran Thanh Van K., Toubart P., Cousson A., Darvill A., Gollin D., Chelf P., and Albersheim P., 1985, Manipulation of the morphogenetic pathways of tobacco expiants by oligosaccharides. Nature, Vol. 314, 6012:615.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Kiêm Tran Thanh Van
    • 1
  • Michel Zivy
    • 2
  • Alain Cousson
    • 1
  • Hervé Thiellement
    • 2
  1. 1.Institut de Physiologie VégétaleCNRSGif sur YvetteFrance
  2. 2.Laboratoire de Génétique des Systèmes VégétauxCNRS-INRA-UPSGif sur YvetteFrance

Personalised recommendations