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Plant Aging pp 199-205 | Cite as

Changes in Carbohydrate Metabolism During Transient Senescence of Mustard (Sinapis Alba L.) Cotyledons

  • H. I. Kasemir
Part of the NATO ASI Series book series (NSSA, volume 186)

Abstract

Higher plants very often display a transient senescence which occurs under unfavorable environmental conditions (for example in stress situations such as light stress or nitrogen deficiency). This adaptive senescence can be reversed as soon as the stress conditions cease. In fact, adaptive senescence is a characteristic of the flexibility of the plant with respect to the choice between preserving an organ or giving it up which in turn may depend on the actual source-sink situation (Kelly and Davies, 1988). It can be assumed that this process of “differential sacrifice” takes place even in one and the same organ at the level of organelles by means of different controls of either protein degradation or synthesis. In this case, the metabolic state within the cell may be a decisive tool for regulation (Trippi et al., 1989). By determining the activity of ribulose-1.5-bisphosphate carboxylase/oxygenase (Rubisco, E.C. 4.1.1.39) and, in some experiments, the mRNA of its small subunit (SSU) we have tried to find out with mustard seedlings whether chloroplast degradation during adaptive senescence can be correlated with parameters of carbohydrate metabolism such as the levels of glucose, sucrose, and starch. As an experimental approach we used the reversible transformation of chloroplast into amylo-plasts which can be induced by continuous irradiation (cWL) and accelerated by nitrogen deprivation.

Keywords

Light Stress Rubisco Activity Mustard Seed Unfavorable Environmental Condition Acer Pseudoplatanus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bergmeyer, H.U., 1984, Methods of Enzymatic Analysis, Vol. VI, Metabolites 1: Carbohydrates, Verlag Chemie, Weinheim, pp. 2–10 and 96–103.Google Scholar
  2. Coleman, L.W., Rosen, B.H. and Schwarzbach, S.D., 1988, Preferential loss of chloroplast proteins in nitrogen deficient Euglena, Plant Cell Physiol.. 29:1007.Google Scholar
  3. Kar, M., 1985, The effect of photoperiod on chlorophyll loss and lipid peroxidation in excised senescing rice leaves, J. Plant Physiol., 123:389.CrossRefGoogle Scholar
  4. Kasemir, H., Rosemann, D. and Oelmüller, R., 1988, Changes in ribulose-l,5-bisphosphate carboxylase and its translatable small subunit mRNA levels during senescence of mustard (Sinapis alba) cotyledons, Physiol. Plant., 73:257.Google Scholar
  5. Kelly, M.O. and Davies, P.J., 1988, The control of whole plant senescence, in: CRC Critical Review of Plant Science, Vol. 7, Issue 2, pp. 139–173.Google Scholar
  6. Luna, C.M. and Trippi, V.S., 1986, Membrane Permeability — Regulation by exogenous sugars during senescence of oat leaf in light and darkness, Plant Cell Physiol., 27:1051.Google Scholar
  7. Ngernprasirtsiri, J., Harinasut, P., Macherel, D., Strzalka, K., Takabe, T., Akazawa, T. and Kojima, K., 1988, Isolation and characterization of the amyloplast envelope-membrane from cultured white-wild cells of sycamore (Acer pseudoplatanus L.), Plant Physiol., 87:371.PubMedCrossRefGoogle Scholar
  8. Ngernprasirtsiri, J., Takabe, T. and Äkazawa, T., 1989, Immunochemical analysis shows that an ATP/ADP-translocator is assosiated with the inner-envelope membranes of amy-loplasts from Acer pseudoplatanus L., Plant Physiol., 89:1024.PubMedCrossRefGoogle Scholar
  9. Trippi, V.S. and De Luca d’Oro, G.M., 1985, The senescence process in oat leaves and its regulation by oxygen concentration and light irradiance, Plant Cell Physiol.. 26:1303.Google Scholar
  10. Trippi, V.S., Gidrol, X. and Pradet, A., 1989, Effects of oxidative stress caused by oxygen and hydrogen peroxide on energy metabolism and senescence in oat leaves, Plant Cell Physiol., 30:157.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • H. I. Kasemir
    • 1
  1. 1.Biologisches Institut IIFreiburgGermany

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