Improvement of Micropropagation Methods Linked To Biochemical Properties During “In Vitro” Cultures
In vitro culture is nowadays an important biotechnology method making possible plant micropropagation and creation of new varieties of economic interest. Our laboratory was one of the first in which these techniques were developed, under Gautheret’s impetus. At the beginning, the main interest of in vitro culture methods was essentially a fundamental one, as it offers new possibilities for tissues to be studied in absence of correlations with the plant and to investigate the morphogenic properties of the cell. This last point remains of value, because it offers the possibility to regenerate a whole plant from isolated and in vitro rejuvenated cells able again to produce meristematic tissue and shooting apices. Recent research in this area is naturally devoted to biochemical markers of morphogenic potentialities of cultured cells and tissues, but a new field of investigation is gaining ground, namely the study of variability of the hereditary structures during development and of the control of genetic expression. As a matter of fact, if stable enough alterations of the hereditary information, both nuclear and cytoplasmic, occur in certain cellular strains, the plants originated from these cells will keep the trace.
KeywordsMaize Chlorophyll Carbohydrate Proline Vasil
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- 1-.L. Velayandom, A.M. Hirsch and D. Fortune-1985-Propagation du Kiwi, Actinidia chinensis L. Planchon, par microbouturage in vitro de nœuds-C.R. Acad. Sci. Paris, série III, n°12, 597–600.Google Scholar
- 2-.M. Monnier, O. Faure and A. Sigogneau-1989-Somatic embryogenesis of Vitis-I.A.P.T.C.-The impact of biotechnology in Agriculture, July 10–12th Amiens, France.Google Scholar
- 3-.R. Heller-1953-Recherches sur la nutrition minérale des tissus végétaux cultivés in vitro-Ann. Sc. Nat., 14, 1–223.Google Scholar
- 5-.B.K. Tripathi and R. Saussay-1980-Sur la multiplication végétative de l’Actinidia chinensis L. Planchon (chinese gooseberry) par culture de racines issues de filet staminaux-C.R. Acad. Sci. Paris, 291, 1067–1069.Google Scholar
- 7-.A.M. Hirsch, D. Fortune and P. Blanchet-1989-Study of diœcism in kiwifruit, Actinidia deliciosa Chevalier-Acta Hort., under press.Google Scholar
- 8-.A.M. Hirsch and D. Fortune-1984-Peroxidase activity and isoperoxidase composition in cultured stem tissue callus and cell suspension of Actinidia chinensis-Z. Pflanzenphysiol., 113, 129–139.Google Scholar
- 9-.B. Verschoore-Martouzet-1985-Etude de la variation topophysique au cours du clonage de Sequoia sempervirens (Endlicher)-Thèse-Paris.Google Scholar
- 10-.C. Moncousin-1982-Peroxidase as tracer for rooting improvement and expressing regeneration during in vitro multiplication of Cynara cardunculus-In Fujiwara A.-Ed. Plant tissue culture-Jap. Assoc. Plant Tissue Culture, Tokyo.Google Scholar
- 16-.E. Sanchez de Jimenez, M. Vargas, R. Aguilar and E. Jimenez-1988-Age dependent responsiveness to cell differentiation stimulus in maize callus culture-Plant. Physiol. Biochem., 26(6), 723–732.Google Scholar
- 23-.W. Halperin-1986-Attainment and retention of morphogenic capacity in vitro-In “Cell culture and somatic cell genetics of plants”, vol.3, I.K. Vasil Ed., p.3–47, Acad. Press New York.Google Scholar