Improvement of Micropropagation Methods Linked To Biochemical Properties During “In Vitro” Cultures
In vitro culture is nowadays an important biotechnology method making possible plant micropropagation and creation of new varieties of economic interest. Our laboratory was one of the first in which these techniques were developed, under Gautheret’s impetus. At the beginning, the main interest of in vitro culture methods was essentially a fundamental one, as it offers new possibilities for tissues to be studied in absence of correlations with the plant and to investigate the morphogenic properties of the cell. This last point remains of value, because it offers the possibility to regenerate a whole plant from isolated and in vitro rejuvenated cells able again to produce meristematic tissue and shooting apices. Recent research in this area is naturally devoted to biochemical markers of morphogenic potentialities of cultured cells and tissues, but a new field of investigation is gaining ground, namely the study of variability of the hereditary structures during development and of the control of genetic expression. As a matter of fact, if stable enough alterations of the hereditary information, both nuclear and cytoplasmic, occur in certain cellular strains, the plants originated from these cells will keep the trace.
KeywordsSomatic Embryogenesis Somaclonal Variation Embryogenic Tissue Staminate Flower Pistillate Flower
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