Plant Aging pp 133-137 | Cite as

Vitrification in Plant Tissue Culture

  • P.-L. Pasqualetto
Part of the NATO ASI Series book series (NSSA, volume 186)


Vitrification is a serious problem since it can affect shoot multiplication and culture vigour and can impede the successful transfer of micropropagated plants to in vivo conditions (Hammerschlag, 1986), Affected plants exhibit tissue hyperhydricity and hypertrophy, chlorophyll a and b deficiency, lack of cell wall lignification or only a layer surrounding the cell membrane, and leaves with large intercellular spaces in the spongy mesophyll and palisade cells. Since the phenomenon apparently involves many factors (gelling agent type, hormones, organic and inorganic compounds, water potential, growth room temperature and light, ecology of container), I will consider morphological, phisiological and ecological aspects separately.


Shoot Proliferation Spongy Mesophyll Cell Wall Lignification Large Intercellular Space Apple Shoot 
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • P.-L. Pasqualetto
    • 1
  1. 1.Azienda Agricola Meristema SRLLaboratorio di micropropagazioneCascine di Buti (Pisa)Italy

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