Plant Aging pp 89-95 | Cite as

In Vitro Micromultiplication of Grapevine: Effect of age, genotype and culture conditions on induction of callus in Vitis spp. leaf segments

  • K. A. Roubelakis-Angelakis
  • K. C. Katsirdakis
Part of the NATO ASI Series book series (NSSA, volume 186)


Stable callus with embryogenic or organogenic potentiality leading to plant regeneration is a prerequisite for in vitro selection and genetic transformation of plants. Krul and Worley (1977) obtained callogenesis from internodes, petioles, flower parts and leaf veins of 2 Vitis vinifera cultivars and 2 interspecific hybrids using Murashige and Skoog (MS, 1962) medium supplemented with 1 mg/l 2,4-D and 0.1 mg/l 6-BAP. Also, Hawker et al. (1973) and Jona and Webb (1978) induced callogenesis in immature grape tissue. Recently, Katsirdakis and Roubelakis-Angelakis (1990) studied the quantitative and qualitative callogenic response of leaf segments in 2 Vitis vinifera cultivars and 6 Vitis spp. interspesific hybrids to 2 culture media and 48 combinations of plant growth regulators. Best results in callus quality were found at 2 or 4 µM 6-BAP in combination with 5 µM NAA. In contrary, 5 or 10 µM 2,4-D and 2 or 4 µM 6-BAP with 5 µM NAA gave callus of poor quality. NAA alone caused development of root radicles. There was a varying response among genotypes (Katsirdakis and Roubelakis-Angelakis, 1990). Maximum quantity of callus from internode expiants of an interspecific hybrid was obtained at 1/5/5 µM, BA/2,4-D/NOA, respectively (Rajasekaran and Mullins, 1981).


Somatic Embryogenesis Interspecific Hybrid Leaf Explants Leaf Segment Shoot Proliferation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alleweldt, G., and Radler, F., 1962, Inter-relationship between photoperiodic behavior of grapes and growth of plant tissue cultures. Plant Physiol., 37(3): 376–379.PubMedCrossRefGoogle Scholar
  2. Barlass, M., and Skene, K.G.M., 1980, Studies on the fragmented shoot apex of grapevine. II. Factors affecting growth and differentiation. J. Exp. Bot., 31 (121): 489–495.CrossRefGoogle Scholar
  3. Brezeanu, A., Iordan, M., and Rosu, A., 1980, The micropropagation of callus from tissue of somatic origin. Rev. Roum. Biol. Ser. Biol. Vegetale, 25: 135–142.Google Scholar
  4. Goussard, P.G., 1981, Effects of cytokinins on elongation, proliferation and total mass of shoots derived from shoot apices of grapevine cultured in vitro. Vitis 20: 228–234.Google Scholar
  5. Hawker, J.S., Downton, W.J.S., Wiskich, D., and Mullins, M.G., 1973, Callus and cell culture from grape berries. Hort. Science, 8(5): 398–399.Google Scholar
  6. Jona, R., and Webb, K.J., 1978, Callus and axillary bud culture of Vitis vinifera Sylvaner Riesling. Sci. Hort., 9(1): 55–60.CrossRefGoogle Scholar
  7. Katsirdakis, K.C., and Roubelakis-Angelakis, 1990, Organo- and callogenic potentiality of leaf segments from Vitis spp. genotypes. J. Amer. Soc. Hort. Sci., accepted.Google Scholar
  8. Ketel, D.H., Bretelez, H., and de Groot, B., 1985, Effect of expiant origin on growth and differentiation of calli from Tagetes species. J. Plant Physiol., 118: 327–333.CrossRefGoogle Scholar
  9. Krul, W.R., and Worley, J.F., 1977, Formation of adventitious embryos in callus cultures of Seyval french hybrid grape. J. Amer. Soc. Hort. Sci., 102(3): 360–363.Google Scholar
  10. Krul, W.R., and Mowbray, G.H., 1984, Grapes, In: Hand Book of Plant Cell Culture, W.R. Sharp, D.A. Evans, P.V. Ammirato, and Y. Yamada, eds, Vol. 2, pp. 396–434, Acad. Press, London.Google Scholar
  11. Krul, W.R., 1988, Recent advances in protoplast culture of horticultural crops: small fruits. Sci. Hortic, 37: 231–246.CrossRefGoogle Scholar
  12. Monette, P.L., 1983, Influence of size of culture vessel on in vitro proliferation of grape in a liquid medium. Plant Cell Tissue Organ Cult., 2: 327–332.CrossRefGoogle Scholar
  13. Murashige, T., and Skoog, F., 1962, A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant., 15: 473–497.CrossRefGoogle Scholar
  14. Nitsch, C., and Nitsen, J.P., 1967, The induction of flowering in vitro in stem segments of Plumbage indica L.I. The production of vegetative buds. Planta 72:355–370.CrossRefGoogle Scholar
  15. Novak, F.J., and Junova, Z., 1983, Clonal propagation of grapevine through in vitro axillary bud culture. Sci. Hort., 18:321–340.CrossRefGoogle Scholar
  16. Rajasekaran, K., and Mullins, M.G., 1981, Organogenesis in internode expiants of grapevines. Vitis 20:218–227.Google Scholar
  17. Reisch, B.I., 1986, Influence of genotype and cytokinins on in vitro shoot proliferation of grapes. J. Amer. Soc. Hort. Sci., 111(1): 138–141.Google Scholar
  18. Roubelakis-Angelakis, K.A., and S.A. Zivanovitch, 1990, Morphogenetic responses of grapevine (Vitis spp.) genotypes to plant growth regulators and culture media. J. Amer. Soc. Hort. Sci., accepted.Google Scholar
  19. Sasahara, H., Tada, K., Irs, M., Takazawa, T., and Tazaki, M., 1981, Regeneration of plantlets by meristem tip culture for virus-free grapevine. J. Japan. Soc. Hort. Sci., 50:169–175.CrossRefGoogle Scholar
  20. Wang, Y., Ge, K., Zou, G., Yang, J., and Yel, M., 1984, Callus induction and plantlet regeneration in grapevines. Int. Symp. on Genetic Manipulation in Crops, Beijing, People’s Republic of China, Abstr., 22–26 Oct. 1984.Google Scholar
  21. Yu, Danhua, and Meredith, C.P., 1986, The influence of expiant origin on tissue browning and shoot production in shoot tip cultures of grapevine. J. Amer. Soc. Hort. Sci., 111 (6): 972–975.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • K. A. Roubelakis-Angelakis
    • 1
  • K. C. Katsirdakis
    • 1
  1. 1.Department of Biology, and Instititute of Molecular Biology and BiotechnologyUniversity of CreteHeraklionGreece

Personalised recommendations