In Vitro Micromultiplication of Grapevine: Effect of age, genotype and culture conditions on induction of callus in Vitis spp. leaf segments
Stable callus with embryogenic or organogenic potentiality leading to plant regeneration is a prerequisite for in vitro selection and genetic transformation of plants. Krul and Worley (1977) obtained callogenesis from internodes, petioles, flower parts and leaf veins of 2 Vitis vinifera cultivars and 2 interspecific hybrids using Murashige and Skoog (MS, 1962) medium supplemented with 1 mg/l 2,4-D and 0.1 mg/l 6-BAP. Also, Hawker et al. (1973) and Jona and Webb (1978) induced callogenesis in immature grape tissue. Recently, Katsirdakis and Roubelakis-Angelakis (1990) studied the quantitative and qualitative callogenic response of leaf segments in 2 Vitis vinifera cultivars and 6 Vitis spp. interspesific hybrids to 2 culture media and 48 combinations of plant growth regulators. Best results in callus quality were found at 2 or 4 µM 6-BAP in combination with 5 µM NAA. In contrary, 5 or 10 µM 2,4-D and 2 or 4 µM 6-BAP with 5 µM NAA gave callus of poor quality. NAA alone caused development of root radicles. There was a varying response among genotypes (Katsirdakis and Roubelakis-Angelakis, 1990). Maximum quantity of callus from internode expiants of an interspecific hybrid was obtained at 1/5/5 µM, BA/2,4-D/NOA, respectively (Rajasekaran and Mullins, 1981).
KeywordsSomatic Embryogenesis Interspecific Hybrid Leaf Explants Leaf Segment Shoot Proliferation
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