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Plant Aging pp 89-95 | Cite as

In Vitro Micromultiplication of Grapevine: Effect of age, genotype and culture conditions on induction of callus in Vitis spp. leaf segments

  • K. A. Roubelakis-Angelakis
  • K. C. Katsirdakis
Part of the NATO ASI Series book series (NSSA, volume 186)

Abstract

Stable callus with embryogenic or organogenic potentiality leading to plant regeneration is a prerequisite for in vitro selection and genetic transformation of plants. Krul and Worley (1977) obtained callogenesis from internodes, petioles, flower parts and leaf veins of 2 Vitis vinifera cultivars and 2 interspecific hybrids using Murashige and Skoog (MS, 1962) medium supplemented with 1 mg/l 2,4-D and 0.1 mg/l 6-BAP. Also, Hawker et al. (1973) and Jona and Webb (1978) induced callogenesis in immature grape tissue. Recently, Katsirdakis and Roubelakis-Angelakis (1990) studied the quantitative and qualitative callogenic response of leaf segments in 2 Vitis vinifera cultivars and 6 Vitis spp. interspesific hybrids to 2 culture media and 48 combinations of plant growth regulators. Best results in callus quality were found at 2 or 4 µM 6-BAP in combination with 5 µM NAA. In contrary, 5 or 10 µM 2,4-D and 2 or 4 µM 6-BAP with 5 µM NAA gave callus of poor quality. NAA alone caused development of root radicles. There was a varying response among genotypes (Katsirdakis and Roubelakis-Angelakis, 1990). Maximum quantity of callus from internode expiants of an interspecific hybrid was obtained at 1/5/5 µM, BA/2,4-D/NOA, respectively (Rajasekaran and Mullins, 1981).

Keywords

Somatic Embryogenesis Interspecific Hybrid Leaf Explants Leaf Segment Shoot Proliferation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • K. A. Roubelakis-Angelakis
    • 1
  • K. C. Katsirdakis
    • 1
  1. 1.Department of Biology, and Instititute of Molecular Biology and BiotechnologyUniversity of CreteHeraklionGreece

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