Structure of the Human cDNAs and Genes Coding for Calbindin D28K and Calretinin
Calbindin D28K was first described by Wasserman and Taylor (1966) and is now considered as the main direct molecular effect of vitamin D derived hormones on gut epithelial cells. It is thought to play a major role in the absorption of calcium from the intestinal lumen and from the distal convoluted tubule of the kidney. The protein is postulated to act as a calcium ferry, facilitating the transport of calcium ions through the cell and keeping the actual free calcium below its toxic level (Jande et al., 1981, McBurney and Neering, 1987). Other cell types containing high amounts of calbindin are the neurons of the central and peripheral nervous systems (Roth et al. 1981), as well as the connected sensory organs (Verstappen et al. 1986), and the alpha and beta cells of the pancreatic islets (Pochet et al. 1987).
KeywordsDistal Convoluted Tubule Restriction Fragment Length Polymorphism Calbindin D28K Chicken Sequence Imperfect Palindrome
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- Huynh, T.V., Young, RA, and Davis, R.W., 1985, Constructing and screening cDNA libraries in gt10 and gt11. in: “DNA cloning techniques”, D.M. Glover, ed., pp. 49–78, IRL Press, Oxford.Google Scholar
- Kimura, M., 1983, “The Neutral Theory of Evolution” Cambridge University Press, Cambridge.Google Scholar
- Nussinov, R., Owens, J., and Maizel, J.V., 1986, Sequence signals in eukaryotic upstream regions. Bioch. Bioph. Acta. 866:109–119.Google Scholar
- Parmentier, M., De Vijlder, J.J.M., Muir, E., Szpirer, C., Islam, M.Q.,Geurts van Kessel, A., Lawson, D.E.M., and Vassart, G., 1989, The human calbindin 27 kDa gene: structural organization of the 5’ and 3’ regions, chromosomal assignment, and restriction fragment length polymorphism. Genomics. 4: 309–319.PubMedCrossRefGoogle Scholar