Comparison Between Rat Brain Calbindin- and Calretinin-Immuno-reactivities

  • A. Résibois
  • F. Blachier
  • J. H. Rogers
  • D. E. M. Lawson
  • R. Pochet
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 269)


Several calcium-binding proteins are present in the central nervous system including the closely related protein calbindin-D 28K (Taylor, 1974; Baimbridge et al., 1982), and calretinin (Rogers, 1987). The existence of calretinin became apparent with the demonstration by immunoblotting that rat cerebral extracts contained two proteins cross-reacting with calbindin antiserum (Pochet et al. 1985). The two proteins differed in size with one being about 2 kDa larger than the other. Subsequently, calretinin was cloned and sequenced (Rogers, 1987) and appeared to be identical to the larger protein recognized by anti-calbindin. Because of the high degree of homology between calbindin and calretinin (Rogers, 1987; Wilson et al., 1988 and Parmentier, 1989), antiserum against either protein may cross-react with the other and therefore the immunohistochemical mapping in brain must be re-assessed. The existence of a single immunoreactive protein band in gels made from some brain extracts does not rule out the possibility that calbindin antiserum used recognized calretinin. Indeed, calretinin-like immunoreactivity is negative in large rat brain areas such as cerebral cortex, most parts of the thalamus and hippocampus (Rogers et al. 1989). A single protein band immunoreactive for calbindin antiserum may thus mean lack of calretinin rather than no cross-immunoreactivity. Cross reactivity between calretinin antiserum and calbindin is easier to check because calbindin-like immunoreactivity is present nearly everywhere in the rat brain.


Inferior Colliculus Auditory Pathway Cyanogen Bromide Medial Nucleus Single Protein Band 
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • A. Résibois
    • 1
  • F. Blachier
    • 2
  • J. H. Rogers
    • 3
  • D. E. M. Lawson
    • 4
  • R. Pochet
    • 1
  1. 1.Laboratoire d’Histologie, Faculté de MédecineUniversité Libre de BruxellesBruxellesBelgique
  2. 2.Laboratoire de Médecine Expérimentale, Faculté de MédecineUniversité Libre de BruxellesBruxellesBelgique
  3. 3.Physiological LabUniversity of CambridgeCambridgeUK
  4. 4.Institute of Animal Physiology and Genetics ResearchCambridgeUK

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