Immunohistochemical Detection of 28KDa Calbindin in Human Tissues

  • Roberto Buffa
  • Paolo Mare
  • Maurizio Salvadore
  • Ambrogio Gini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 269)


We recently investigated (Buffa et al., 1989 ) tissues from some avians (quail, duck and chicken) and some mammals (rat, guinea pig, cat and dog) in order to localize the cellular source of 28KDa calbindin, using a polyclonal antiserum raised against a chicken antigen. Endocrine cells reacting selectively with this calbindin antiserum were found in the anterior lobe of the pituitary, in the adrenal medulla, and in the gastric mucosa of all mammalian species examined, reactivity was also found in the parafollicular cells from the mammalians studied and from the duck thyroid, in some insular cells from the pancreas of both mammals and avians and in the endocrine cells from the rectal mucosa of quails. Our results are in accordance with those reported in the literature (Roth et al., 1982; Kondo et al., 1085, Pochet et al., 1987). Although some species variability was observed in the distribution of the immünoreactivity, we found rather unexpectedly, that most of the endocrine cells from human adult tissues failed to react with the calbindin antiserum from the chicken. These finding may be either related to technical problems (e.g. overfixation, selective influence of fixatives) or to subtle antigenic differences (Baimbridge et al., 1982; Sonnenberg et al., 1984) between chicken and human calbindins.


Endocrine Cell Thyroid Medullary Carcinoma Pancreatic Polypeptide Medullary Carcinoma Oxyntic Mucosa 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baimbridge, R.G., Miller, J.T, and Parker, CO., 1982, Calcium-binding protein distribution in the rat brain. Brain Res., 239:519.PubMedCrossRefGoogle Scholar
  2. Buffa, R., Mare’, P., Salvadore, M., Solcia, E., Furness, J.B., and Lawson, D.E.M., 1989, Calbindin 28KDa in endocrine cells of known or putative calcium-regulating function. Histochemistry, 91:107.PubMedCrossRefGoogle Scholar
  3. Care, A.D., Bruce, J.B., Bodklins, J., Kennedy, A.D., Conaway, H., and Anast, C.S., 1971, Role of pancreozimin-cholecystokinin and structurally related compounds as calcitonin secretagogues. En docrinology. 89:262.Google Scholar
  4. Copp, D.H., 1964, Parathyroid, calcitonin and control of plasma calcium. Recent Progr. Horm. Res., 20:59.PubMedGoogle Scholar
  5. Fischer, J A., Blum, G.N., and Binswanger, V. 1973, Parathyroid hormone response to epinephrine: in vivo J. Clin. Invest., S2:2434.Google Scholar
  6. Hakanson, R., Bottcher, G., Ekblad, E., Panula, P., Simonsson, M., and Dohlsten, M., 1986, Histamine in endocrine cells of the stomach. A survey of several species using a panel of Histamine antibodies. Histochemistry. 86:5.PubMedCrossRefGoogle Scholar
  7. Jande, S.S., Maler, L., and Lawson, D.E.M., 1981, Immunohistochemical mapping of vitamin D -dependent calcium-binding protein in brain. Nature. 294:765.PubMedCrossRefGoogle Scholar
  8. Jande, S.S., Tolnai, S., and Lawson, D.E.M.,1981, Immunohistochemical localization of vitamin D -dependent calcium-binding protein in duodenum, kidney, uterus and cerebellum of chickens. Histochemistry. 71:99.PubMedCrossRefGoogle Scholar
  9. Kondo, H., Kuramoto, H., Iwanaga, T., and Fujita, T., 1985, Cerebellar Purkinje cell-specific protein like immunoreactivity in nor-adrenalin-chromaffin cells and ganglion cells but not in adrenalin-chromaffin cells in the rat adrenal medulla. Arch. Histol. Jpn., 48:421.PubMedCrossRefGoogle Scholar
  10. Levy, F.O., Eikvar, L., Froysa, A., Cervenka, J., Yoganathan, T., and Hansson, V., 1985, Testicular Calcitriol receptors; appearance during development and presence in adult testicular cell cultures. Vitamin D. A Chemical,Biochemical and Clinical Update pp. 151, 1985 Walter de Gruyter &Co, Berlin.Google Scholar
  11. Pochet, R., Pipeleers, D.G., and Malaisse, W.J., 1987, Calbindin 27KDa: preferential localization in non B-islet cells of the rat pancreas. Biol. Cell., 61:155.PubMedCrossRefGoogle Scholar
  12. Polak, J.M., and Van Noorden, S., 1983, Immunocytochemistry: applications in pathology and biology. In: Immunocytochemistry today. Techniques and practice. J.M. Polak, and S. Van Noorden, eds., J. Wright, London.Google Scholar
  13. Rindi, G., Buffa, R., Sessa, F., Tortora, O., and Solcia, E., 1986, Chromogranin A,B, and C Immunoreac-tivities of mammalian endocrine cells.Distribution,distinction from costored hormones/prohormones and relationship with the argyrophil component of the secretory granules. Histochemistry. 85:19.PubMedCrossRefGoogle Scholar
  14. Roth, J., Bonner-Weir, S., Norman, A.V., and Orci, L., 1982, Immunocytochemistry of vitamin D-dependent calcium-binding protein in chick pamcreas: exclusive localization in B-cells. Endocrinology 110:2216.PubMedCrossRefGoogle Scholar
  15. Schreiner, D.S., Jande, S.S., and Lawson, D.E.M., 1986, Immunocytochemical localization of vitamin D de-pendent calcium-binding protein. Brain Res., 222:452.Google Scholar
  16. Schreiner, D.S., Jande, S.S., Parkers, CO., Lawson, D.E.M., and Thomasset, M., 1983, Immunocytochemical demonstration of two vitamin D-dependent calcium-binding proteins in mammalian kidney. Acta Anat., 117:1.PubMedCrossRefGoogle Scholar
  17. Schulak, J.A., and Kaplan, E.L., 1975, The importance of the stomach in gastrin-induced hypocalcemia in the rat. Endocrinology. 96:1217.PubMedCrossRefGoogle Scholar
  18. Sonnenberg, J., Pansini, A.R., and Christakos, S., 1984, Vitamin D dependent rat renal calcium-binding protein : development of a radioimmunoassay, tissue distribution and immunologic identification. Endocrinology. 115:640.PubMedCrossRefGoogle Scholar
  19. Spencer, R., Chapman, M., Emtage, J.S., and Lawson, D.E.M., 1978, Production and properties of vitamin D induced mRNA from chick calcium-binding protein. Eur. J.Biochem., 71:393.Google Scholar
  20. Stumpf, W.E., and O’Brien, L.P., 1987, Autoradiographic studies with 3H,1–25 dihydroxy vitamin D3 in thyroid and associated tissues of the neck region. Histochemistry, 87:53.PubMedCrossRefGoogle Scholar
  21. Wilson, P.W., Rogers, J., Harding, M., Pohl, V., Pattyn, G., and Lawson, D.E.M., 1988, Structure of chick chromosomal genes for Calbindin and Calretinin. J. Mol. Biol. 200:615.PubMedCrossRefGoogle Scholar
  22. Windeck, R., Brown, E.M., Gardner, D.G., and Aurbach, G.D., 1978, Effect of gastrointestinal hormones on isolated bovine parathyroid cells. Endocrinology. 103:2020.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Roberto Buffa
    • 1
  • Paolo Mare
    • 1
  • Maurizio Salvadore
    • 1
  • Ambrogio Gini
    • 1
  1. 1.Dipartimento di Patologia Umana ed Ereditaria, Sezione di Anatomia Patologica 2Universita’ di PaviaVareseItalia

Personalised recommendations