Abstract
We recently investigated (Buffa et al., 1989 ) tissues from some avians (quail, duck and chicken) and some mammals (rat, guinea pig, cat and dog) in order to localize the cellular source of 28KDa calbindin, using a polyclonal antiserum raised against a chicken antigen. Endocrine cells reacting selectively with this calbindin antiserum were found in the anterior lobe of the pituitary, in the adrenal medulla, and in the gastric mucosa of all mammalian species examined, reactivity was also found in the parafollicular cells from the mammalians studied and from the duck thyroid, in some insular cells from the pancreas of both mammals and avians and in the endocrine cells from the rectal mucosa of quails. Our results are in accordance with those reported in the literature (Roth et al., 1982; Kondo et al., 1085, Pochet et al., 1987). Although some species variability was observed in the distribution of the immünoreactivity, we found rather unexpectedly, that most of the endocrine cells from human adult tissues failed to react with the calbindin antiserum from the chicken. These finding may be either related to technical problems (e.g. overfixation, selective influence of fixatives) or to subtle antigenic differences (Baimbridge et al., 1982; Sonnenberg et al., 1984) between chicken and human calbindins.
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© 1990 Plenum Press, New York
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Buffa, R., Mare, P., Salvadore, M., Gini, A. (1990). Immunohistochemical Detection of 28KDa Calbindin in Human Tissues. In: Pochet, R., Lawson, D.E.M., Heizmann, C.W. (eds) Calcium Binding Proteins in Normal and Transformed Cells. Advances in Experimental Medicine and Biology, vol 269. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5754-4_33
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DOI: https://doi.org/10.1007/978-1-4684-5754-4_33
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